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Time-resolved studies of singlet-oxygen emission from L1210 leukemia cells labeled with 5-(N-hexadecanoyl)amino eosin. A comparison with a one-dimensional model of singlet-oxygen diffusion and quenching.

Abstract
Time-resolved measurements were made of near-infrared emission from 5-(N-hexadecanoyl)amino-eosin-labeled L1210 leukemia cells following pulsed-laser excitation. The cells were suspended in phosphate-buffered saline made with deuterium oxide solvent. A significant fraction of the emission occurring 10-80 microseconds after the laser pulse was due to singlet oxygen. This singlet-oxygen emission is believed to result from singlet oxygen generated near the cell-membrane surface, where 5-(N-hexadecanoyl)amino eosin is known to concentrate, and then diffusing out into the buffer. The intensity and the kinetics of the experimentally observed singlet-oxygen emission were in excellent agreement with the predictions of a theoretical one-dimensional model of singlet-oxygen diffusion and quenching. During the 10-80 microseconds time period studied, most of the singlet oxygen was located in the buffer. Thus, the use of water-soluble singlet-oxygen quenchers, such as histidine, provide one means of separating the singlet-oxygen emission from other sources of light during this time interval.
AuthorsA Baker, J R Kanofsky
JournalPhotochemistry and photobiology (Photochem Photobiol) Vol. 57 Issue 4 Pg. 720-7 (Apr 1993) ISSN: 0031-8655 [Print] United States
PMID7685124 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Photosensitizing Agents
  • 5-(N-hexadecanoyl)aminoeosin
  • Singlet Oxygen
  • Histidine
  • Carnosine
  • Ascorbic Acid
  • Oxygen
  • Eosine Yellowish-(YS)
Topics
  • Animals
  • Ascorbic Acid (pharmacology)
  • Carnosine (pharmacology)
  • Cell Membrane (metabolism)
  • Diffusion
  • Eosine Yellowish-(YS) (analogs & derivatives, metabolism, pharmacology)
  • Histidine (pharmacology)
  • Kinetics
  • Leukemia L1210 (metabolism)
  • Mice
  • Models, Biological
  • Oxygen (metabolism)
  • Photochemistry
  • Photosensitizing Agents (pharmacology)
  • Singlet Oxygen
  • Time Factors
  • Tumor Cells, Cultured

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