Effect of TYB-2285 on histamine release from human basophils in the presence/absence of IL-3.

Abstract

1. The present study was carried out to investigate the effect of TYB-2285 (3,5-bis(acetoxyacetylamino)-4-chlorobenzonitrile) on histamine release from human basophils upon stimulation with anti-IgE in the presence/absence of recombinant interleukin 3 (rIL-3). 2. Histamine release was enhanced by the pretreatment of basophils with rIL-3 (0.3-10 U/ml) in 18 out of 22 subjects. 3. Histamine release in the presence of rIL-3 was inhibited by TYB-2285 and disodium cromoglycate (DSCG) in a dose-dependent manner (1-100 microM). 4. Histamine release in the absence of rIL-3 was not inhibited by TYB-2285 or DSCG at concentrations less than 1 mM. 5. From these results, it is suggested that TYB-2285 and DSCG might regulate allergic inflammation by the suppression of mediator release enhanced by IL-3.

• Authors: T Tominaga, A Watanabe, H Hayashi, Y Yanagihara, A Koda
• Journal: General pharmacology (Gen Pharmacol) Vol. 24 Issue 2 Pg. 345-8 (Mar 1993) ISSN: 0306-3623 [Print] England
• PMID: 7683300 (Publication Type: Journal Article)

Chemical References

• Antibodies, Anti-Idiotypic
• Interleukin-3
• Nitriles
• Recombinant Proteins
• anti-IgE antibodies
• 3,5-bis(acetoxyacetylamino)-4-chlorobenzonitrile
• Cromolyn Sodium

Topics

• Antibodies, Anti-Idiotypic (immunology)
• Basophils (drug effects, metabolism)
• Cromolyn Sodium (pharmacology)
• Dose-Response Relationship, Drug
• Histamine Release (drug effects)
• Humans
• In Vitro Techniques
• Interleukin-3 (pharmacology)
• Nitriles (pharmacology)
• Recombinant Proteins (pharmacology)