Abstract | BACKGROUND: The objective of this study was to characterize the acute and chronic inflammation induced by the intramural injection of peptidoglycan- polysaccharide (PG-PS) into the distal colon of genetically susceptible rats. METHODS: RESULTS: Intramural injection of PG-PS into the distal colon produced a local biphasic inflammatory response composed of an acute episode 3 days after injection; this was followed by a spontaneous reactivation of chronic granulomatous colitis manifested by colonic thickening, adhesions, and infiltration of the submucosa and muscularis propria with macrophages, neutrophils, and lymphocytes at 3-4 weeks. Mucosal ulcers were evident only at 3 weeks, but hepatic nodules, splenic necrosis, and arthritis were evident at both 3 and 4 weeks after PG-PS injection. PG-PS produced significant increases in colonic mucosal permeability, myeloperoxidase activity, and plasma nitrite and nitrate levels at 3 weeks postinjection compared with controls. PG-PS stimulated the production of nitrite by elicited peritoneal macrophages and neutrophils in vitro. CONCLUSIONS:
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Authors | T Yamada, R B Sartor, S Marshall, R D Specian, M B Grisham |
Journal | Gastroenterology
(Gastroenterology)
Vol. 104
Issue 3
Pg. 759-71
(Mar 1993)
ISSN: 0016-5085 [Print] United States |
PMID | 7680014
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Peptidoglycan
- Peroxidase
- Nitric Oxide Synthase
- Amino Acid Oxidoreductases
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Topics |
- Amino Acid Oxidoreductases
(biosynthesis)
- Animals
- Chronic Disease
- Colitis
(chemically induced, metabolism, pathology)
- Crohn Disease
(etiology)
- Disease Models, Animal
- Enzyme Induction
- Female
- Granuloma
(chemically induced, metabolism, pathology)
- Intestinal Mucosa
(metabolism, pathology)
- Nitric Oxide Synthase
- Peptidoglycan
- Peroxidase
(metabolism)
- Rats
- Rats, Inbred Lew
- Streptococcus pyogenes
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