Characterization of SFP2, a putative sulfate permease gene of Saccharomyces cerevisiae.

Abstract	The SFP2 gene of Saccharomyces cerevisiae has been characterized. The deduced amino acid sequence contained twelve highly hydrophobic domains and showed 50, 47, 44 and 48% homologies to Neurospora crassa sulfate permease II (CYS14), soybean GMAK170 nodulin, human colon mucosa protein (DRA) and a putative open reading frame (ORF) downstream of Escherichia coli prs (phosphoribosyl pyrophosphatate synthetase) gene, respectively, in the aligned regions. Cells lacking SFP2 were viable and displayed no obvious decrease in their growth rate. Southern blot analysis revealed that SFP2 exists as a single copy in haploid genome. Northern blot analysis showed that SFP2 produced a 2.8-kb transcript which was highly expressed under sulfur derepressing condition. SFP2 mRNA was found to turn over with a half-life of approximately 15 min, which may contribute to the regulation of sulfate permease function, and reached its maximal level in about 22 h after depression.
Authors	Y H Jin, Y K Jang, M J Kim, M R Rad, L Kirchrath, R H Seong, S H Hong, C P Hollenberg, S D Park
Journal	Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 214 Issue 2 Pg. 709-15 (Sep 14 1995) ISSN: 0006-291X [Print] United States
PMID	7677785 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Anion Transport Proteins DNA Primers Membrane Transport Proteins Recombinant Proteins sulfate permease
Topics	Amino Acid Sequence Anion Transport Proteins Base Sequence Blotting, Northern Cloning, Molecular DNA Primers Escherichia coli (enzymology, genetics) Genes, Fungal Humans Membrane Transport Proteins (biosynthesis, chemistry, genetics) Molecular Sequence Data Mutagenesis Neurospora crassa (enzymology, genetics) Polymerase Chain Reaction Recombinant Proteins (biosynthesis, chemistry) Restriction Mapping Saccharomyces cerevisiae (enzymology, genetics) Sequence Homology, Amino Acid Glycine max (genetics) Transcription, Genetic

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