Korkormicins, novel depsipeptide antitumor antibiotics from Micromonospora sp C39500: fermentation, precursor directed biosynthesis and biological activities.

Abstract

Micromonospora sp C39500, isolated in our laboratory from a soil sample, produced a complex of seven novel depsipeptide antitumor antibiotics, designated korkormicins. The major component of the complex, korkormicin A, has a MW of 1452 and a molecular formula of C66H84N16O22. Korkormicin A exhibits potent in vivo antitumor activity against P388 leukemia and M109 lung carcinoma implanted intraperitoneally (ip) in mice, with effective doses of 0.05-0.20 mg kg-1 injection-1, for five or three ip injections, respectively. It is also active against Gram-positive bacteria but inactive against Gram-negative bacteria. The production of korkormicin A was enhanced by 3-fold when 0.1% L-valine was added to the production culture at 48 h. A titer of 401.0 micrograms ml-1 was achieved in the fermenter culture supplemented with 0.1% L-valine.

• Authors: K S Lam, D R Gustavson, G A Hesler, T T Dabrah, J A Matson, R L Berry, W C Rose, S Forenza
• Journal: Journal of industrial microbiology (J Ind Microbiol) Vol. 15 Issue 1 Pg. 60-5 (Jul 1995) ISSN: 0169-4146 [Print] England
• PMID: 7662300 (Publication Type: Journal Article)

Chemical References

• Antibiotics, Antineoplastic
• Culture Media
• Valine
• Nitrogen

Topics

• Animals
• Antibiotics, Antineoplastic (biosynthesis, pharmacology)
• Carcinoma (drug therapy)
• Culture Media (chemistry)
• Drug Screening Assays, Antitumor
• Female
• Fermentation (drug effects, physiology)
• Leukemia P388 (drug therapy)
• Lung Neoplasms (drug therapy)
• Mice
• Mice, Inbred BALB C
• Mice, Inbred DBA
• Micromonospora (classification, drug effects, metabolism)
• Molecular Weight
• Neoplasm Transplantation
• Nitrogen (pharmacology)
• Valine (pharmacology)