Internalization of E. coli ST mediated by guanylyl cyclase C in T84 human colon carcinoma cells.

Abstract	Internalization of Escherichia coli heat-stable enterotoxin (ST) mediated by guanylyl cyclase C was examined in T84 human colon carcinoma cells. Surface-associated, receptor-bound ST was quantitatively separated from intracellular ligand employing acidic guanidine-HCl. ST was internalized in a time-, temperature-, and ligand concentration-dependent fashion only by cells specifically expressing guanylyl cyclase C. Only receptors which bound reversibly to ST appeared to mediate endocytosis. The rate of internalization of ST empirically determined in these studies was 0.23 min-1. The density of surface receptors for ST was similar at 4 degrees C and 37 degrees C, suggesting that these receptors recycle back to the cell surface following internalization of ligand. Similarly, internalized ST was rapidly cleared from the intracellular compartment following endocytosis. These studies demonstrate that ST undergoes ligand-dependent receptor-mediated endocytosis in human colon carcinoma cells.
Authors	R Urbanski, S L Carrithers, S A Waldman
Journal	Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1245 Issue 1 Pg. 29-36 (Aug 17 1995) ISSN: 0006-3002 [Print] Netherlands
PMID	7654763 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References	Bacterial Toxins Enterotoxins Escherichia coli Proteins Iodine Radioisotopes Receptors, Cell Surface Receptors, Peptide heat stable toxin (E coli) Guanylate Cyclase Receptors, Enterotoxin Receptors, Guanylate Cyclase-Coupled
Topics	Amino Acid Sequence Bacterial Toxins (metabolism) Consensus Sequence Endocytosis Enterotoxins (metabolism) Escherichia coli Escherichia coli Proteins Guanylate Cyclase (metabolism) Humans Iodine Radioisotopes Kinetics Molecular Sequence Data Receptors, Cell Surface (metabolism) Receptors, Enterotoxin Receptors, Guanylate Cyclase-Coupled Receptors, Peptide (metabolism) Temperature Tumor Cells, Cultured

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