Molecular mimicry and ankylosing spondylitis: possible role of a novel sequence in pullulanase of Klebsiella pneumoniae.

Abstract

Molecular mimicry has been shown between two sequences of Klebsiella pneumoniae pulD secretion protein (DRDE) with HLA-B27 (DRED) and pulA (pullulanase) enzyme (Gly-X-Pro) with types I, III and IV collagen respectively. IgG antibody levels in AS patients were elevated against 16mer synthetic peptides of HLA-B27 and pulD by enzyme immunosorbent assay (ELISA) compared to controls (P < 0.001). ELISA assays against K. pneumoniae grown in the absence and presence of pullulan demonstrated significant levels of IgA antibody in AS patients compared to controls (P < 0.001). Increased IgA and IgG antibody levels to pulA and types I and IV collagen were observed in AS patients compared to controls (P < 0.001). These observations could be relevant in the sequence of molecular events in AS.

• Authors: M Fielder, S J Pirt, I Tarpey, C Wilson, P Cunningham, C Ettelaie, A Binder, S Bansal, A Ebringer
• Journal: FEBS letters (FEBS Lett) Vol. 369 Issue 2-3 Pg. 243-8 (Aug 07 1995) ISSN: 0014-5793 [Print] England
• PMID: 7649265 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Bacterial
• Bacterial Outer Membrane Proteins
• Glucans
• HLA-B27 Antigen
• Immunoglobulin G
• Peptides
• PulD protein, Klebsiella pneumoniae
• pullulan
• Collagen
• Glycoside Hydrolases
• pullulanase

Topics

• Amino Acid Sequence
• Antibodies, Bacterial (blood)
• Bacterial Outer Membrane Proteins (genetics, immunology)
• Collagen (genetics)
• Cross Reactions
• Enzyme-Linked Immunosorbent Assay
• Glucans
• Glycoside Hydrolases (genetics)
• HLA-B27 Antigen (genetics, immunology)
• Humans
• Immunoglobulin G (blood)
• Klebsiella pneumoniae (enzymology, genetics, immunology)
• Molecular Mimicry
• Molecular Sequence Data
• Peptides (chemical synthesis, immunology)
• Sequence Homology, Amino Acid
• Spondylitis, Ankylosing (genetics, immunology, microbiology)