HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Purification and N-terminal sequence of a novel interferon-induced 100 kD protein secreted by mouse Ehrlich ascites tumor cells.

Abstract
Mouse Ehrlich ascites tumor cells secrete a 100 kD protein when they are stimulated by murine interferon-beta (2000 IU/ml). This 100 kD protein was purified from conditioned medium by chromatography on phenyl-Sepharose CL-4B, phosphocellulose, DEAE-Sephacel, and Vydac C4 columns and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A single species of 100 kD protein was isolated, and its N-terminal sequence analysis suggested that mouse Ehrlich ascites tumor cell-derived interferon-induced 100 kD protein is a novel protein.
AuthorsS Tominaga, J Utsumi
JournalJournal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research (J Interferon Cytokine Res) Vol. 15 Issue 5 Pg. 417-20 (May 1995) ISSN: 1079-9907 [Print] UNITED STATES
PMID7648443 (Publication Type: Journal Article)
Chemical References
  • Culture Media, Conditioned
  • Neoplasm Proteins
  • Recombinant Proteins
  • Interferon-beta
Topics
  • Amino Acid Sequence
  • Animals
  • Carcinoma, Ehrlich Tumor (metabolism)
  • Chromatography, High Pressure Liquid
  • Culture Media, Conditioned
  • Electrophoresis, Polyacrylamide Gel
  • Interferon-beta (pharmacology)
  • Mice
  • Molecular Sequence Data
  • Neoplasm Proteins (isolation & purification, metabolism)
  • Recombinant Proteins (pharmacology)
  • Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: