Targeted disruption of the mouse factor VIII gene produces a model of haemophilia A.

Abstract

Haemophilia A is a classic X-linked disease which affects 1 in 5-10,000 males in all populations and is caused by defects in coagulation factor VIII. Roughly 60% of patients have severe disease with factor VIII activity < 1% of normal; they have frequent spontaneous bleeding into joints, soft tissues, muscles and internal organs. These patients usually require regular injections of plasma-derived or recombinant human factor VIII. Because this is expensive and can potentially lead to life-threatening complications, other forms of therapy, including gene therapy, have been proposed. Natural canine models of factor VIII and factor IX deficiency have been available for many years, and gene therapy attempts on these dogs have met with partial success. However, a small animal model of the disease is desirable for studies of factor VIII function and gene therapy. Using gene targeting, we have made a mouse with severe factor VIII deficiency.

• Authors: L Bi, A M Lawler, S E Antonarakis, K A High, J D Gearhart, H H Kazazian Jr
• Journal: Nature genetics (Nat Genet) Vol. 10 Issue 1 Pg. 119-21 (May 1995) ISSN: 1061-4036 [Print] United States
• PMID: 7647782 (Publication Type: Letter, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Factor VIII

Topics

• Animals
• Base Sequence
• Blotting, Southern
• Cell Line
• Cloning, Molecular
• Disease Models, Animal
• Exons
• Factor VIII (genetics)
• Female
• Hemophilia A (genetics)
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Molecular Sequence Data
• Mutagenesis, Insertional
• Polymerase Chain Reaction