Redistribution of
vitamin E in the rat body was studied during
acute pancreatitis induced by two intraperitoneal doses of
cerulein 40 micrograms/kg of
body weight at 1-hr intervals.
Hyperamylasemia (2064 +/- 521 vs 6419 +/- 129 U/dL) and pancreatic
edema (pancreatic water content, 71 +/- 1.2% vs 78 +/- 2%) were observed. In this model the increased level of
lipid soluble fluorophore was also observed (274 +/- 18 vs 120 +/- 9.0 relative fluorescence per g dry wt). Parallel with these changes was a decrease in the level of
vitamin E in the serum and an increase in the pancreas. The concentration of
vitamin E in the pancreas after 6 h was 162 +/- 8.5 ng/mg dry mass vs 128.1 +/- 6.1 ng/mg dry mass in control animals. The effect of
heparin on
vitamin E redistribution induced by
acute pancreatitis was also investigated. It was found that
heparin at a dose of 100 U/kg body mass prevents the drop of the
vitamin E level in the serum as well as the increases in the concentration in the pancreas tissue. It was concluded that
acute pancreatitis induced redistribution of
vitamin E in the rat body. Moreover, we studied the effects of
heparin treatment on oxidative stress in the pancreas tissue.
Acute pancreatitis caused an increase in
lipofuscin accumulation, and a decrease in
protein sulfhydryl groups in
citrate synthetase (CS) and in
malate dehydrogenase (MDH) activity.
Heparin treatment that protected
vitamin E accumulation in the pancreas tissue did not influence the changes in the level of
lipofuscin and
proteins sulfhydryl.(ABSTRACT TRUNCATED AT 250 WORDS)