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Differential up-regulation of HLA class I molecules on neuronal and glial cell lines by virus infection correlates with differential induction of IFN-beta.

Abstract
Adult neurons normally lack the expression of MHC class I molecules, which has implications on virus clearance from the central nervous system. The author previously demonstrated that HLA class I up-regulation in measles virus (MV)-infected glial cells is primarily mediated by IFN-beta. In contrast, this study demonstrates that MV-infection of the neuronal cell lines IMR-32 and CHP-126 fails to up-regulate HLA class I expression, which was associated with an inability of MV to induce IFN-beta in the neuronal cell lines. However, treatment with IFN-beta on coculture of the IMR-32 neuronal cell line with MV-infected glioma cells resulted in the up-regulation of HLA class I on the former, which could be neutralized by anti-IFN-beta Ab. The inability of MV to up-regulate HLA class I expression on the neuronal cell line IMR-32 was not virus specific because similar findings were observed with mumps virus or stimulation with the synthetic dsRNA polyinosinic polycytidylic acid (PIPC). Induction of IFN-beta gene expression by virus requires binding of NF-kappa B to the positive regulatory domain II element of the IFN-beta promoter. Our studies indicate that MV, TNF-alpha, or PIPC induces NF-kappa B (p50 and p65 subunits) binding to positive regulatory domain II in the glioma cell line. In contrast, such activity was induced by TNF-alpha but not MV or PIPC in the neuronal cell line IMR-32. This indicated that HLA class I expression is differentially regulated in glial and neuronal cell lines in response to MV, which correlates with differential binding of NF-kappa B to the IFN-beta promoter and induction of IFN-beta gene expression.
AuthorsS S Dhib-Jalbut, Q Xia, P D Drew, P T Swoveland
JournalJournal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 155 Issue 4 Pg. 2096-108 (Aug 15 1995) ISSN: 0022-1767 [Print] United States
PMID7636259 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Histocompatibility Antigens Class I
  • NF-kappa B
  • RNA, Messenger
  • Interferon-beta
  • Poly I-C
Topics
  • Base Sequence
  • Histocompatibility Antigens Class I (biosynthesis)
  • Humans
  • Interferon-beta (biosynthesis, genetics)
  • Measles virus (physiology)
  • Molecular Sequence Data
  • Mumps virus (physiology)
  • NF-kappa B (metabolism)
  • Neuroglia (immunology)
  • Neurons (immunology)
  • Poly I-C (pharmacology)
  • RNA, Messenger (analysis)
  • Tumor Cells, Cultured
  • Up-Regulation

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