Hemolytic activity of the Pasteurella haemolytica leukotoxin.

Abstract

A Pasteurella haemolytica mutant incapable of producing leukotoxin was created by allelic replacement. Concentrated culture supernatants from wild-type P. haemolytica, but not from the mutant, contained the 102-kDa leukotoxin protein and lysed bovine lymphoma cells and sheep erythrocytes. Wild-type P. haemolytica demonstrated the typical beta-hemolytic phenotype on sheep and rabbit blood agar, whereas the mutant did not.

• Authors: G L Murphy, L C Whitworth, K D Clinkenbeard, P A Clinkenbeard
• Journal: Infection and immunity (Infect Immun) Vol. 63 Issue 8 Pg. 3209-12 (Aug 1995) ISSN: 0019-9567 [Print] United States
• PMID: 7622250 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Bacterial Toxins
• DNA, Bacterial
• Exotoxins
• Hemolysin Proteins
• leukotoxin

Topics

• Animals
• Bacterial Toxins (toxicity)
• DNA, Bacterial (genetics)
• Exotoxins (toxicity)
• Genes, Bacterial
• Hemolysin Proteins
• In Vitro Techniques
• Mannheimia haemolytica (pathogenicity)
• Mutagenesis
• Rabbits
• Sheep