HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Active immunization with recombinant V antigen from Yersinia pestis protects mice against plague.

Abstract
The gene encoding V antigen from Yersinia pestis was cloned into the plasmid expression vector pGEX-5X-2. When electroporated into Escherichia coli JM109, the recombinant expressed V antigen as a stable fusion protein with glutathione S-transferase. The glutathione S-transferase-V fusion protein was isolated from recombinant E. coli and cleaved with factor Xa to yield purified V antigen as a stable product. Recombinant V antigen was inoculated intraperitoneally into mice and shown to induce a protective immune response against a subcutaneous challenge with 3.74 x 10(6) CFU of virulent Y. pestis. Protection correlated with the induction of a high titer of serum antibodies and a T-cell response specific for recombinant V antigen. These results indicate that V antigen should be a major component of an improved vaccine for plague.
AuthorsS E Leary, E D Williamson, K F Griffin, P Russell, S M Eley, R W Titball
JournalInfection and immunity (Infect Immun) Vol. 63 Issue 8 Pg. 2854-8 (Aug 1995) ISSN: 0019-9567 [Print] United States
PMID7622205 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Bacterial
  • Antigens, Bacterial
  • DNA Primers
  • LcrV protein, Yersinia
  • Pore Forming Cytotoxic Proteins
  • Recombinant Fusion Proteins
  • Vaccines, Synthetic
Topics
  • Animals
  • Antibodies, Bacterial (biosynthesis)
  • Antigens, Bacterial (immunology)
  • Base Sequence
  • DNA Primers (chemistry)
  • Female
  • Genes, Bacterial
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Plague (prevention & control)
  • Pore Forming Cytotoxic Proteins
  • Recombinant Fusion Proteins
  • Vaccines, Synthetic
  • Yersinia pestis (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: