Induction of rat liver microsomal epoxide hydrolase by its endogenous substrate 16 alpha, 17 alpha-epoxyestra-1,3,5-trien-3-ol.

Abstract

1. The influence of the endogenous steroid epoxides 16 alpha, 17 alpha-epoxyestra-1,3,5(10)-trien-3-ol (estroxide) and 16 alpha, 17 alpha-expoxiandrost-4-en-3-one (androstene oxide) and their metabolic precursors estra-1,3,5(10), 16-tetraen-3-ol (estratetraenol) and androsta-4, 16-dien-3-one (androstadienone) on the specific activities of hepatic microsomal and soluble epoxide hydrolase, glutathione S-transferase, dihydrodiol dehydrogenase, and 7-ethoxycoumarin deethylase was investigated in the male Sprague-Dawley rat. 2. Both estroxide and estratetraenol induced microsomal epoxide hydrolase activity towards styrene oxide and estroxide itself 2-2.5-fold and glutathione conjugation of 1-chloro-2,4-dinitrobenzene (CDNB) 1.6-fold after intraperitoneal administration of a high dose of compound (300 mg per kg of body weight). 3. In addition, estroxide decreased 7-ethoxycoumarin deethylation down to 20% of the activity observed in the untreated rat, whereas estratetraenol enhanced the activity of soluble epoxide hydrolase towards trans-stilbene oxide by a factor of 1.7. 4. In contrast, neither androstene oxide nor androstadienone showed a significant influence on any of the parameters under investigation. Dihydrodiol dehydrogenase was not significantly changed by any of the treatments.

• Authors: F Fändrich, B Degiuli, U Vogel-Bindel, M Arand, F Oesch
• Journal: Xenobiotica; the fate of foreign compounds in biological systems (Xenobiotica) Vol. 25 Issue 3 Pg. 239-44 (Mar 1995) ISSN: 0049-8254 [Print] England
• PMID: 7618350 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Epoxy Compounds
• 16 beta,17 beta-epoxy-1,3,5(10)-estratrien-3-ol
• styrene oxide
• Oxidoreductases
• 7-Alkoxycoumarin O-Dealkylase
• trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
• Glutathione Transferase
• Epoxide Hydrolases
• Estriol

Topics

• 7-Alkoxycoumarin O-Dealkylase (metabolism)
• Animals
• Enzyme Induction (drug effects)
• Epoxide Hydrolases (biosynthesis, metabolism)
• Epoxy Compounds (metabolism)
• Estriol (analogs & derivatives, metabolism, pharmacology)
• Glutathione Transferase (metabolism)
• Male
• Microsomes, Liver (enzymology)
• Oxidoreductases (metabolism)
• Rats
• Rats, Sprague-Dawley