Abstract |
Streptolysin O-permeabilized cells incubated with a high concentration (5-10 mg/ml) of cytosolic proteins and ATP-generating system exhibit redistribution into the endoplasmic reticulum (ER) of Golgi integral proteins ( mannosidase II, galactosyltransferase, TGN 38), detected by immunofluorescence. In addition, mannosidase II is detected in the ER of cells exposed to a high concentration of cytosolic proteins and processed for immunolectron microscopy by immunoperoxidase. The redistribution process requires ATP and is not affected by previous microtubule depolymerization. Ultrastructural observations indicate that Golgi disassembly occurs by budding of coated vesicles. This stage of the process is inhibited by GTP-gamma S, AIF(3-5), transducin beta gamma subunits, and mastoparan, indicating the involvement of trimeric G proteins. At a later stage, vesicles lose their coats and fuse with the ER. This part of the process does not occur in cells incubated at either 15 degrees C or 20 degrees C, or exposed to N-ethylmaleimide. In cells treated with either cholera or pertussis toxin Golgi redistribution into the ER shows a 50-fold lower requirement for cytosolic factors than in untreated cells. These data suggest a regulatory role for both alpha s and alpha i trimeric G proteins in the normal Golgi-ER retrograde transport taking place in intact cells.
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Authors | J Hidalgo, M Muñiz, A Velasco |
Journal | Journal of cell science
(J Cell Sci)
Vol. 108 ( Pt 4)
Pg. 1805-15
(Apr 1995)
ISSN: 0021-9533 [Print] England |
PMID | 7615693
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bacterial Proteins
- Glycoproteins
- Macromolecular Substances
- Membrane Glycoproteins
- Membrane Proteins
- Streptolysins
- TGOLN2 protein, human
- Tgoln2 protein, rat
- streptolysin O
- Adenosine Triphosphate
- Galactosyltransferases
- Mannosidases
- mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase
- GTP-Binding Proteins
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Topics |
- Adenosine Triphosphate
(metabolism)
- Animals
- Bacterial Proteins
- Cell Line
- Cell Membrane Permeability
- Cytosol
(physiology)
- Endoplasmic Reticulum
(metabolism, ultrastructure)
- Fluorescent Antibody Technique
- GTP-Binding Proteins
(chemistry, metabolism)
- Galactosyltransferases
(analysis, metabolism)
- Glycoproteins
- Golgi Apparatus
(metabolism, ultrastructure)
- HeLa Cells
- Humans
- Kidney
- Kinetics
- Macromolecular Substances
- Mannosidases
(analysis, metabolism)
- Membrane Glycoproteins
(analysis, metabolism)
- Membrane Proteins
- Microscopy, Electron
- Microscopy, Immunoelectron
- Rats
- Streptolysins
- Time Factors
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