Induction of heat-shock protein 72 protects against ischemia/reperfusion in rat small intestine.

Abstract

BACKGROUND & AIMS:

Induction of heat-shock protein 72 is associated with enhanced tolerance to subsequent nonthermal stresses. This study evaluated whether induction of heat-shock protein 72 protects against intestinal ischemia/reperfusion injury.

METHODS:

Groups of nonheated and heated rats underwent sham operation, 30 minutes of ischemia by occlusion of the superior mesenteric artery, or ischemia followed by 60 minutes of reperfusion. Whole-body hyperthermia to a core temperature of 41.5-42 degrees C for 15-20 minutes was followed by passive cooling 2-3 hours before the experiment. Samples of small intestine were obtained for determination of heat-shock protein 72 production and ex vivo generation of prostaglandin E2 and leukotriene B4 and for histological assessment of mucosal injury and number of neutrophils.

RESULTS:

Hyperthermia significantly increased heat-shock protein 72 production and significantly reduced ischemia/reperfusion-induced mucosal injury, neutrophilic infiltration, and leukotriene B4 production. Levels of leukotriene B4 and numbers of neutrophils were well correlated in nonheated (r = 0.72) but not in heated groups (r = -0.16). The elevation of prostaglandin E2 levels in response to ischemia and reperfusion was unaltered by hyperthermia.

CONCLUSIONS:

The mechanism of heat stress-induced protection against intestinal ischemia/reperfusion injury involves inhibition of leukotriene B4 production and subsequent prevention of neutrophil activation and chemotaxis.

• Authors: A Stojadinovic, J Kiang, R Smallridge, R Galloway, T Shea-Donohue
• Journal: Gastroenterology (Gastroenterology) Vol. 109 Issue 2 Pg. 505-15 (Aug 1995) ISSN: 0016-5085 [Print] United States
• PMID: 7615200 (Publication Type: Journal Article)

Chemical References

• HSP72 Heat-Shock Proteins
• Heat-Shock Proteins
• Leukotriene B4

Topics

• Animals
• Blood Pressure (physiology)
• Fever
• HSP72 Heat-Shock Proteins
• Heart Rate (physiology)
• Heat-Shock Proteins (biosynthesis)
• Intestinal Mucosa (blood supply, metabolism, pathology)
• Intestine, Small (blood supply, metabolism, pathology)
• Leukotriene B4 (biosynthesis)
• Male
• Neutrophils (physiology)
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (metabolism, pathology, prevention & control)