Induction of programmed cell death in human breast cancer cells by an unsymmetrically alkylated polyamine analogue.

Abstract

The need for antineoplastic compounds with novel mechanisms of action is great. One such agent is the recently synthesized polyamine analogue N1-ethyl-N11-((cyclopropyl)methyl)-4,8-diazaundecane (CPENSpm). Exposure of hormone-dependent and -independent human breast cancer cells to 0.1-10 microM CPENSpm led to both growth inhibition and induction of programmed cell death. Fragmentation of DNA to high molecular weight fragments and oligonucleosomal-sized fragments, both characteristic of programmed cell death, was determined to be time and concentration dependent. Depletion of natural polyamine pools and accumulation of the analogue was also demonstrated. These data provide the first evidence that a polyamine analogue induces programmed cell death.

• Authors: D E McCloskey, R A Casero Jr, P M Woster, N E Davidson
• Journal: Cancer research (Cancer Res) Vol. 55 Issue 15 Pg. 3233-6 (Aug 01 1995) ISSN: 0008-5472 [Print] United States
• PMID: 7614453 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antineoplastic Agents
• DNA, Neoplasm
• Polyamines
• RNA, Messenger
• N(1)-ethyl-N-(11)-((cyclopropyl)methyl)-4,8-diazaundecane-1,11-diamine
• Acetyltransferases
• diamine N-acetyltransferase

Topics

• Acetyltransferases (analysis)
• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects, physiology)
• Breast Neoplasms (enzymology, pathology, physiopathology)
• Cell Division (drug effects)
• DNA, Neoplasm (drug effects)
• Humans
• Neoplasms, Hormone-Dependent (enzymology, pathology, physiopathology)
• Polyamines (pharmacology)
• RNA, Messenger (analysis)
• Tumor Cells, Cultured