Abstract |
Serial extraction study of Alzheimer's disease (AD) and control brains revealed 4, 3.7 and 3 kD amyloid beta protein (A beta) species accumulated in AD brains. In the fractions extracted with TBS, 10% SDS and formic acid, considerable amounts of A beta species were recovered in SDS fractions besides TBS and formic acid fractions. Immunoblotting with several site-specific antibodies confirmed not only the presence of 4 kD A beta starting at the first amino acid of A beta but also 2 smaller A beta species with modification of their amino-termini in the highly resolutional Tris/ Tricine gel system. A beta solubility using these solvents was associated with both modification of the amino-terminus and length of carboxyl-terminus of A beta. Especially, a large amount of modified A beta was found to be accumulated as forms with different solubility in AD brains.
|
Authors | Y Harigaya, M Shoji, T Kawarabayashi, M Kanai, T Nakamura, T Iizuka, Y Igeta, T C Saido, N Sahara, H Mori |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 211
Issue 3
Pg. 1015-22
(Jun 26 1995)
ISSN: 0006-291X [Print] United States |
PMID | 7598687
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Amyloid beta-Peptides
- Antibodies, Monoclonal
|
Topics |
- Aged
- Aged, 80 and over
- Alzheimer Disease
- Amyloid beta-Peptides
(chemistry, immunology)
- Antibodies, Monoclonal
- Antibody Specificity
- Brain Chemistry
- Fluoroimmunoassay
- Humans
- Immunoblotting
|