Abstract |
Effect of triethyllead on the specific [3H] flunitrazepam binding was studied in rat cortical and cerebellar P2 fractions in vitro and in tissue homogenates of several rat brain regions ex vivo after 5 daily subcutaneous doses of 1.9 mg/kg triethyllead acetate to rats. Up to concentration of 100 microM, triethyllead did not affect significantly the specific [3H] flunitrazepam binding but attenuated marginally (14-18%) the GABAA receptor agonist, muscimol-induced elevation of [3H] flunitrazepam binding in cerebellar tissue. After the subacute treatment of rats with triethyllead, the specific [3H] flunitrazepam binding was 27% lower in cerebellum compared to control animals. In other brain regions the receptor binding was not changed. The data suggest that triethyllead modified the cerebellar GABAA receptor complex causing decreased binding in the benzodiazepine site. Such an inhibitory effect in the GABAA receptor complex may decrease cerebellar inhibitory output and augment the triethyllead induced convulsions and tremor.
|
Authors | H Komulainen, A Keränen, V Saano |
Journal | European journal of pharmacology
(Eur J Pharmacol)
Vol. 293
Issue 2
Pg. 167-71
(Jul 01 1995)
ISSN: 0014-2999 [Print] Netherlands |
PMID | 7589231
(Publication Type: Journal Article)
|
Chemical References |
- GABA-A Receptor Antagonists
- Nerve Tissue Proteins
- Organometallic Compounds
- Receptors, GABA-A
- Muscimol
- Flunitrazepam
- triethyllead
|
Topics |
- Animals
- Binding, Competitive
(drug effects)
- Cerebellum
(drug effects, metabolism)
- Cerebral Cortex
(drug effects, metabolism)
- Flunitrazepam
(metabolism)
- GABA-A Receptor Antagonists
- In Vitro Techniques
- Male
- Muscimol
(metabolism)
- Nerve Tissue Proteins
(metabolism)
- Organometallic Compounds
(metabolism, toxicity)
- Rats
- Rats, Wistar
- Receptors, GABA-A
(drug effects)
|