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Improved high-performance liquid chromatographic method for the determination of polyamines as their benzoylated derivatives: application to P388 cancer cells.

Abstract
A simple reversed-phase HPLC method was developed for the determination of eight polyamines or monoacetylpolyamines, as their benzoylated derivatives. Interfering products, inherent to the benzoyl chloride derivatization technique (benzoic acid, methyl benzoate and benzoic anhydride), were identified. A new derivatization procedure for their total elimination was developed without any loss of sensitivity and selectivity. Not only the HPLC method was validated, but also the choice of an internal standard was investigated. The results show that it is possible to use this HPLC assay to determine the polyamine content in P388 cancer cells. Furthermore, the method is now being used to evaluate the uptake of various polyamines by P388 cancer cells and by other cancer and parasitic cells.
AuthorsE Schenkel, V Berlaimont, J Dubois, M Helson-Cambier, M Hanocq
JournalJournal of chromatography. B, Biomedical applications (J Chromatogr B Biomed Appl) Vol. 668 Issue 2 Pg. 189-97 (Jun 23 1995) ISSN: 1572-6495 [Print] Netherlands
PMID7581854 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Benzoates
  • Polyamines
  • Solutions
  • Benzoic Acid
Topics
  • Animals
  • Benzoates (analysis, chemistry)
  • Benzoic Acid
  • Chromatography, High Pressure Liquid (methods)
  • Kinetics
  • Leukemia P388
  • Polyamines (analysis, chemistry)
  • Reproducibility of Results
  • Solutions
  • Spectrophotometry, Ultraviolet
  • Tumor Cells, Cultured

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