The nucleotide sequence of a transposable haloalkanoic acid dehalogenase regulatory gene (dehRI) from Pseudomonas putida strain PP3 and its relationship with sigma 54-dependent activators.

Abstract

The mobile genetic element, DEH found in Pseudomonas putida PP3 carries a 2-haloalkanoic acid dehalogenase structural gene, dehI, and its associated regulatory gene, dehRI. The nucleotide sequence of dehRI was determined. The gene had an open reading frame putatively encoding for a 64 kDa protein containing 571 amino acid residues. The protein was similar to previously published sequences of several other sigma 54-dependent activator proteins. Amino acid sequence analysis showed that the deduced DehRI protein clustered with the NifA nitrogenase regulatory activator family, and was most closely related, with 47.7% similarity, to a 'NifA-like' deduced partial sequence from a plasmid-encoded ORF in Pseudomonas sp. strain NS671, associated with L-amino acid production. The domain structure of DehRI was analysed by alignment with other NifA-like and NtrC-like sequences and showed a highly conserved central region of approximately 230 amino acids, and a potential DNA-binding domain. No homology was detected between the deduced DehRI and other sigma 54-dependent activator sequences at the N-terminus, a result which was consistent with that region being the domain which recognised inducer.

• Authors: A W Topping, A W Thomas, J H Slater, A J Weightman
• Journal: Biodegradation (Biodegradation) Vol. 6 Issue 3 Pg. 247-55 (Sep 1995) ISSN: 0923-9820 [Print] Netherlands
• PMID: 7579999 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Proteins
• DNA Transposable Elements
• DNA-Binding Proteins
• DehRI protein, Pseudomonas putida
• Sigma Factor
• Transcription Factors
• DNA-Directed RNA Polymerases
• RNA Polymerase Sigma 54
• Hydrolases
• 2-haloacid dehalogenase

Topics

• Amino Acid Sequence
• Bacterial Proteins
• Base Sequence
• DNA Transposable Elements
• DNA-Binding Proteins
• DNA-Directed RNA Polymerases (chemistry)
• Gene Expression Regulation, Bacterial
• Genes, Bacterial
• Genes, Regulator
• Hydrolases (genetics)
• Molecular Sequence Data
• Open Reading Frames
• Pseudomonas putida (enzymology, genetics)
• RNA Polymerase Sigma 54
• Sequence Alignment
• Sequence Analysis, DNA
• Sequence Homology, Amino Acid
• Sigma Factor (chemistry)
• Transcription Factors (chemistry, genetics)