Photoimmobilization of a bioactive laminin fragment and pattern-guided selective neuronal cell attachment.

Abstract

To attain light-dependent functionalization of biocompatible materials, a photolabel-derivatized, bioactive laminin fragment has been synthesized, chemically characterized, and photoimmobilized. Covalent high-resolution patterning of the laminin fragment CDPGYIGSR to hydroxylated fluorinated ethylene propylene (FEP-OH), poly(vinyl alcohol), and glycophase glass has been achieved. The synthetic peptide CDPGYIGSR was thermochemically coupled to either N-[m-[3-(trifluoromethyl)-diazirin-3-yl]phenyl]-4-maleimidobuty ramide or 4-maleimidobenzophenone. Photolabel-derivatized peptides were radiolabeled, and 20 and 300 microns-sized patterns were visualized by autoradiography. The biospecific interaction of photoimmobilized laminin fragments with cells was investigated by analyzing the selective attachment of NG 108-15 neuroblastoma x glioma cells which bear CDPGYIGSR-specific cell surface receptors. On photopatterned FEP-OH membranes NG 108-15 cells differentiated in serum-supplemented media within 1 day. Specific attachment to the immobilized oligopeptide CDPGYIGSR was assessed in serum-free media with competitive binding studies, showing an 82% decrease in cell adherence after the cell receptors were blocked with soluble CDPGYIGSR.

• Authors: J F Clémence, J P Ranieri, P Aebischer, H Sigrist
• Journal: Bioconjugate chemistry (Bioconjug Chem) 1995 Jul-Aug Vol. 6 Issue 4 Pg. 411-7 ISSN: 1043-1802 [Print] United States
• PMID: 7578361 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Azirines
• Benzophenones
• Indicators and Reagents
• Laminin
• Maleimides
• Peptide Fragments
• Receptors, Cell Surface
• fluorinated ethylene propylene
• N-(2-(3-(trifluoromethyl)diazirin-3-yl)phenyl)-4-maleimidobutyramide
• Polytetrafluoroethylene
• Polyvinyl Alcohol
• benzophenone-4-maleimide

Topics

• Amino Acid Sequence
• Animals
• Azirines
• Benzophenones
• Binding, Competitive
• Cell Adhesion
• Cells, Immobilized
• Glass
• Glioma
• Hybrid Cells
• Indicators and Reagents
• Laminin
• Maleimides
• Molecular Sequence Data
• Molecular Structure
• Neuroblastoma
• Neurons (cytology, physiology)
• Peptide Fragments (chemical synthesis)
• Photochemistry
• Polytetrafluoroethylene (analogs & derivatives)
• Polyvinyl Alcohol
• Receptors, Cell Surface (physiology)