Abstract | UNLABELLED: 85 E. coli strains producing various beta-lactamase were studied: TEM low level: n = 11, TEM high level: n = 4, oxacillinase (OXA): n = 5, céphalosporinase (CASE) +/- TEM: n = 5, inhibitor resistant TEM (IRT) n = 50, IRT+TEM: n = 3, IRT+ CASE: n = 7. Their susceptibility to amoxicillin (AMX), amoxicillin+clavulanate (AMC), ticarcillin ( TIC), ticarcillin+clavulanate (TCC), piperacillin (PIP), piperacillin+tazobactam (TAZ), mecillinam (MEC), cephalotin (CF), cefoxitin (FOX) et ceftazidime (CAZ) were evaluated by a disk diffusion method, in order to determine the resistance pattern which allows a reliable detection of IRT-producing strains. The phenotype AMX R, TIC R, AMCI/R, TCCI/R et CFS was observed in 46 out of 53 IRT +/- TEM strains, 4/5 of OXA strains and 1/11 of TEM low level strains. A better sensitivity could be obtained by a modification of breakpoints for AMC, TCC and CF, however OXA-producing strains remain indistinguishable from IRT-strains.
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Authors | J P Romaszko, L Bret, C Henquell, D Sirot, C Chanal, J Sirot |
Journal | Pathologie-biologie
(Pathol Biol (Paris))
Vol. 43
Issue 4
Pg. 306-9
(Apr 1995)
ISSN: 0369-8114 [Print] France |
Vernacular Title | Détection des beta-lactamases résistantes aux inhibiteurs (IRT) par la méthode de diffusion. |
PMID | 7567120
(Publication Type: Journal Article)
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Chemical References |
- Anti-Bacterial Agents
- beta-Lactams
- beta-Lactamases
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Topics |
- Anti-Bacterial Agents
(pharmacology)
- Bacteriological Techniques
- Drug Resistance, Microbial
- Escherichia coli
(drug effects, enzymology)
- Humans
- In Vitro Techniques
- beta-Lactamases
(metabolism)
- beta-Lactams
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