Abstract | BACKGROUND: The diagnostic possibilities of sensitization to various acid anhydrides are limited because of the lack of standardized allergens for the different test systems. This makes the diagnosis of IgE-mediated sensitization caused by occupational exposure difficult. METHODS: We prepared conjugates of human serum albumin with phthalic, maleic, trimellitic, and pyromellitic anhydrides to be used for IgE estimation by enzyme-allergosorbent test, skin prick tests, and nasal and bronchial challenge tests. Nine anhydride workers, who complained of various respiratory symptoms, were studied. RESULTS: Of the nine workers, four had immediate-type skin test responses to one or more conjugates. All four subjects had elevated IgE concentrations in addition to two other workers. Three of six nasal challenges and four of nine bronchial challenges resulted in positive responses. All but one of the positive nasal or bronchial test responses were associated with elevated IgE levels. The seven positive challenge test results included five positive skin test responses. On the other hand, in all but two of the subjects with negative challenge test results, no specific IgE could be detected. In these two subjects the negative results were associated with low levels of IgE, and in one, with the absence of asthma. None of the results of tests with unconjugated anhydrides were positive. CONCLUSIONS:
|
Authors | X Baur, A Czuppon |
Journal | The Journal of allergy and clinical immunology
(J Allergy Clin Immunol)
Vol. 96
Issue 4
Pg. 489-94
(Oct 1995)
ISSN: 0091-6749 [Print] United States |
PMID | 7560660
(Publication Type: Journal Article)
|
Chemical References |
- Anhydrides
- Antibodies
- Serum Albumin
- Immunoglobulin E
- Sodium Chloride
|
Topics |
- Anhydrides
(immunology)
- Antibodies
(analysis)
- Bronchial Provocation Tests
- Chemical Industry
- Drug Hypersensitivity
(diagnosis)
- Humans
- Immunoglobulin E
(immunology)
- Nasal Provocation Tests
- Occupational Diseases
(diagnosis)
- Osmolar Concentration
- Serum Albumin
- Skin Tests
- Sodium Chloride
|