Abstract |
10(7) mouse ascites tumor cells/ml incubated at 37 degrees C in 0.5 to 1.0 X 10(-4) M Janus green B or in 1.0 X 10(-4) M phenazine methosulphate are destroyed in 100 per cent oxygen atmosphere but remain transplantable in nitrogen atmosphere. The "sensitizing" effect of oxygen can be substituted by SH inhibitors ( iodoacetic acid, iodoacetamide and their spinlabelled variants) as well as by some nitroxide free radicals. The " oxygen effect" is blocked by mercaptoethanole or cooling. Compared with the spectrum of native cells a more symmetrical singlet of larger amplitude, approximately g = 2 value, arose in the ESR spectrum of Janus green B treated cells. The " oxygen effect" observed in the presence of Janus green B differs in several ways from the oxygen effect of ionizing radiation and from the "photodynamic" effect.
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Authors | R Holland, G Elek |
Journal | Strahlentherapie
(Strahlentherapie)
Vol. 154
Issue 2
Pg. 127-33
(Feb 1978)
ISSN: 0039-2073 [Print] Germany |
PMID | 75598
(Publication Type: Journal Article)
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Chemical References |
- Coloring Agents
- Iodoacetates
- Nitro Compounds
- Methylphenazonium Methosulfate
- Nitrogen
- Oxygen
- Iodoacetamide
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Topics |
- Animals
- Carcinoma, Ehrlich Tumor
(metabolism)
- Coloring Agents
(toxicity)
- Electron Spin Resonance Spectroscopy
- Iodoacetamide
(toxicity)
- Iodoacetates
(toxicity)
- Methylphenazonium Methosulfate
(toxicity)
- Mice
- Neoplasms, Experimental
(metabolism)
- Nitro Compounds
(toxicity)
- Nitrogen
- Oxygen
(toxicity)
- Staining and Labeling
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