Synthetic octapeptide pyroGlu-Asp-Asp-Ser-Asp-Glu-Glu-Asn promotes differentiation in promyelocytic HL-60 cell line.

Abstract

The activity of the octapeptide pyroGlu-Asp-Asp-Ser-Asp-Glu-Glu-Asn in the control of cell growth and differentiation of human myeloblastic leukemia cells HL-60 is reported. Treatment with peptide slightly slows down the rate of cellular proliferation and this effect becomes more evident in cells grown for several weeks in the presence of the effector. An enhanced effect (40-50% inhibition respect to the control) is found in reversibly permeabilized cells and after 1% DMSO is added to the medium. Moreover the presence of peptide markedly increases the percentage of cells differentiated by DMSO and RA. The effect in DMSO-induced cells is more evident than that observed in RA-induced cells. This in agreement with our hypothesis that DMSO facilitates the peptide entry and its effect is due to an intracellular action.

• Authors: V Marsili, A Angiolillo, G L Gianfranceschi
• Journal: Cellular and molecular biology (Noisy-le-Grand, France) (Cell Mol Biol (Noisy-le-grand)) Vol. 41 Issue 4 Pg. 515-23 (Jun 1995) ISSN: 0145-5680 [Print] France
• PMID: 7549788 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Peptides
• Tretinoin
• Dimethyl Sulfoxide

Topics

• Amino Acid Sequence
• Cell Differentiation (drug effects)
• Cell Division (drug effects)
• Dimethyl Sulfoxide (pharmacology)
• Dose-Response Relationship, Drug
• HL-60 Cells
• Humans
• Molecular Sequence Data
• Peptides (chemical synthesis, chemistry, pharmacology)
• Tretinoin (pharmacology)