| Abstract | We investigated the effects of thyroid status on nitric oxide synthase (NOS) gene expression in the rat hypothalamic paraventricular (PVN) and supraoptic nuclei (SON). Propylthiouracil (PTU)-induced hypothyroidism in male rats produced a highly significant reduction in NOS gene transcripts in the PVN and SON, as assessed by quantitative in situ hybridization histochemistry with a specific oligodeoxynucleotide probe. The addition of T3 (40 micrograms/kg) to the PTU-containing diet completely prevented the reduction in NOS transcripts. Hyperthyroidism, induced by adding 160 micrograms/kg T3 to the food, more than doubled the prevalence of NOS transcripts in the PVN and SON after a similar time. Up-regulation of NOS gene transcripts induced by the osmotic stimulus of chronic salt loading was markedly attenuated by PTU-induced hypothyroidism. These results demonstrate a major effect of thyroid status on regulation of NOS gene expression in the hypothalamus. |
| Authors | Y Ueta, A Levy, H S Chowdrey, S L Lightman
(Affiliation: Department of Medicine, University of Bristol, Bristol Royal Infirmary, United Kingdom.)
|
| Journal | Endocrinology
(Endocrinology)
Vol. 136
Issue 10
Pg. 4182-7
(Oct 1995)
ISSN: 0013-7227 UNITED STATES |
| PMID | 7545100
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- RNA, Messenger
- Thyroid Hormones
- Thyrotropin-Releasing Hormone
- Propylthiouracil
- Thyrotropin
- Nitric Oxide Synthase
- Amino Acid Oxidoreductases
|
| Topics |
- Amino Acid Oxidoreductases
(genetics)
- Animals
- Base Sequence
- Gene Expression Regulation, Enzymologic
(drug effects)
- Hypothalamus
(enzymology)
- Male
- Molecular Sequence Data
- Nitric Oxide Synthase
- Propylthiouracil
(pharmacology)
- RNA, Messenger
(analysis)
- Rats
- Rats, Sprague-Dawley
- Thyroid Hormones
(physiology)
- Thyrotropin
(genetics)
- Thyrotropin-Releasing Hormone
(genetics)
|
