Study of LDL and acetylated LDL endocytosis by mononuclear cells in HIV infection.

Abstract

Activated lymphocytes have a high level of low density lipoprotein (LDL) uptake as compared to resting lymphocytes, whereas scavenger receptors for acetylated LDL (Ac-LDL) are expressed on limited number of immune cells, i.e., monocytes/macrophages. The endocytosis of LDL and Ac-LDL by mononuclear cells was studied during in vitro and in vivo HIV infection, in order to use LDL and Ac-LDL as carriers of antiviral and/or immunomodulatory drugs towards lymphocytes and monocytes. The uptake of LDL and Ac-LDL was analyzed by cytofluorimetry. LDL endocytosis in PHA/IL2-activated lymphocytes was higher than in resting lymphocytes. In vitro HIV infection of PHA/IL2-activated lymphocytes did not alter the high LDL endocytosis in lymphocytes. CD4+ and CD8+ cells. In a group of 12 symptomatic patients there was no alteration of LDL endocytosis in lymphocytes, CD4 and CD8 lymphocytes. In another group of 23 individuals, the Ac-LDL endocytosis mediated by CD14+ monocytes was unaltered in asymptomatic patients (n = 6) and in some symptomatic patients (n = 6, CD14+ cells > 100/mm3). On the contrary, in other symptomatic patients (n = 11, CD14+ cells < 100/mm3), the number of Ac-LDL+ CD14+ cells decreased, whereas their efficiency of Ac-LDL endocytosis increased as compared to those of other HIV+ patients. In conclusion, the use of lipoproteins as carriers to increase the drug delivery to CD4+ lymphocytes and to CD14+ monocytes can be envisaged, since: (i) the LDL endocytosis was not impaired in CD4 lymphocytes of HIV+ patients, and (ii) the Ac-LDL uptake by monocytes was altered only in some patients of stage IV.

• Authors: L Juompan, J Puel, G J Fournié, H Benoist
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1272 Issue 1 Pg. 21-8 (Aug 15 1995) ISSN: 0006-3002 [Print] Netherlands
• PMID: 7545009 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• Antigens, Differentiation, Myelomonocytic
• CD3 Complex
• Cell Adhesion Molecules
• Drug Carriers
• Lipopolysaccharide Receptors
• Lipoproteins, LDL
• Receptors, LDL
• Receptors, Scavenger
• acetyl-LDL

Topics

• Antigens, CD (analysis)
• Antigens, Differentiation, Myelomonocytic (analysis)
• Binding, Competitive
• CD3 Complex (analysis)
• CD4-Positive T-Lymphocytes (immunology, metabolism, virology)
• CD8-Positive T-Lymphocytes (immunology, metabolism, virology)
• Cell Adhesion Molecules
• Cells, Cultured
• Drug Carriers (metabolism)
• Endocytosis (physiology)
• HIV Infections (immunology, metabolism)
• HIV-1 (physiology)
• Humans
• Leukocytes, Mononuclear (immunology, metabolism, virology)
• Lipopolysaccharide Receptors
• Lipoproteins, LDL (metabolism)
• Lymphocyte Activation
• Monocytes (immunology, metabolism, virology)
• Receptors, LDL (metabolism)
• Receptors, Scavenger