Prospective comparison of direct immunofluorescence and conventional staining methods for detection of Giardia and Cryptosporidium spp. in human fecal specimens.

Abstract

In a prospective comparative study, 2,696 consecutive fresh stool specimens over the course of 1 year were examined for Giardia lamblia and Cryptosporidium parvum by using a direct immunofluorescent-monoclonal antibody stain (for unspun specimens) and conventional staining methods (chlorazol black E for Giardia cysts and modified Kinyoun acid-fast for Cryptosporidium oocysts). The direct immunofluorescent-monoclonal antibody method resulted in a significantly increased detection rate for both giardia (118 versus 79 specimens, 49.4%; P = 0.006) and cryptosporidia (39 versus 23 specimens, 69.6%; P = 0.055).

• Authors: A J Alles, M A Waldron, L S Sierra, A R Mattia
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 33 Issue 6 Pg. 1632-4 (Jun 1995) ISSN: 0095-1137 [Print] United States
• PMID: 7544365 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antibodies, Monoclonal

Topics

• Animals
• Antibodies, Monoclonal
• Cryptosporidiosis (diagnosis, parasitology)
• Cryptosporidium (immunology, isolation & purification)
• Evaluation Studies as Topic
• Feces (parasitology)
• Fluorescent Antibody Technique
• Giardia (immunology, isolation & purification)
• Giardiasis (diagnosis, parasitology)
• Humans
• Prospective Studies
• Staining and Labeling (methods)