Abstract |
Endothelin-1, a 21-residue peptide isolated from vascular endothelial cells, has a broad spectrum of actions. To clarify the involvement of endothelin-1 in acute pancreatitis, we examined the effects of endothelin-1 and its receptor antagonist BQ-123 on cerulein-induced pancreatitis in rats. Rats were infused intravenously with heparin-saline (control), endothelin-1 (100 pmol/kg/hr), cerulein (5 micrograms/kg/hr), or cerulein plus endothelin-1 for 3.5 hr. In another experiment, cerulein or cerulein plus BQ-123 (3 mg/kg/hr) was infused. Infusion of cerulein caused hyperamylasemia and pancreatic edema. Endothelin-1, when infused with cerulein, decreased the extent of pancreatic edema with a significant increase in the pancreatic dry- to wet-weight ratio. Histological changes induced by cerulein were markedly attenuated when endothelin-1 was given with cerulein. In contrast, endothelin-receptor blockade with BQ-123 further augmented pancreatic edema caused by cerulein. The extent of inflammatory cell infiltration was greater than BQ-123 was given with cerulein. Endothelin-1 or BQ-123 had no influence on hyperamylasemia. This study suggests that endothelin-1 has protective effects on experimental acute pancreatitis.
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Authors | M Kogire, K Inoue, S Higashide, K Takaori, Y Echigo, Y J Gu, S Sumi, K Uchida, M Imamura |
Journal | Digestive diseases and sciences
(Dig Dis Sci)
Vol. 40
Issue 6
Pg. 1207-12
(Jun 1995)
ISSN: 0163-2116 [Print] United States |
PMID | 7540127
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Endothelin Receptor Antagonists
- Endothelins
- Peptides, Cyclic
- Ceruletide
- Amylases
- cyclo(Trp-Asp-Pro-Val-Leu)
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Topics |
- Acute Disease
- Amylases
(blood, drug effects)
- Analysis of Variance
- Animals
- Ceruletide
- Disease Models, Animal
- Drug Evaluation, Preclinical
- Endothelin Receptor Antagonists
- Endothelins
(blood, therapeutic use)
- Male
- Pancreas
(drug effects, pathology)
- Pancreatitis
(blood, chemically induced, drug therapy, pathology)
- Peptides, Cyclic
(therapeutic use)
- Rats
- Rats, Wistar
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