[Modifications of gene expression by tumor promoters].

Abstract

The modifications of gene expression by tumor promoters were analyzed in vitro and in vivo. The results of slot blot hybridizations showed that tumor promoter TPA induced c-fos and c-myc expressions in mouse fibroblast cell line BALB/3T3 and rat liver, decreased the levels of Rb RNA in BALB/3T3 cell line and of alpha 1-I3 RNA in rat liver. It was also demonstrated that tumor promoter phenobarbital influenced c-fos and c-myc expressions and decreased alpha 1I3 mRNA level in rat liver during a long term experiment. Phenobarbital was found to have no effect on c-fos and c-myc expressions in rat liver during a short experiment. Tumor promoters induced the expressions of c-fos and c-myc which were positively-related to cancer formation and inhibited the expressions of Rb and alpha 1-I3 which were negatively-related to cancer formation. This implied that tumor promotion played an important role in cancer development and tumor promoters exerted their effects selectively according to the attributes of different genes.

• Authors: C Zhang, Q Zhao, S Guo, M Zhao, S Cheng
• Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 17 Issue 1 Pg. 11-5 (Feb 1995) ISSN: 1000-503X [Print] China
• PMID: 7540119 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acute-Phase Proteins
• Carcinogens
• Protease Inhibitors
• alpha 1-inhibitor 3
• RNA
• Croton Oil
• Tetradecanoylphorbol Acetate
• Phenobarbital

Topics

• 3T3 Cells (drug effects, metabolism)
• Acute-Phase Proteins
• Animals
• Carcinogens (toxicity)
• Croton Oil (pharmacology)
• Gene Expression (drug effects)
• Genes, Retinoblastoma
• Genes, fos
• Genes, myc
• Liver (cytology, metabolism)
• Male
• Mice
• Phenobarbital (pharmacology)
• Protease Inhibitors (metabolism)
• RNA (biosynthesis)
• Rats
• Rats, Wistar
• Tetradecanoylphorbol Acetate (toxicity)