HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Molecular analysis of the essential and nonessential genetic elements in the genome of peanut chlorotic streak caulimovirus.

Abstract
The DNA genome of caulimoviruses contains a set of essential genes: I (movement gene), IV (major capsid protein gene), V (reverse transcriptase gene), and VI (gene coding for a post-transcriptional activator of the expression of other virus genes). In peanut chlorotic streak caulimovirus (PCISV), three ORFs, A, B, and C, are located between genes I and IV. They are dissimilar to other caulimovirus ORFs. ORF VII of PCISV is a homolog of ORF VII of soybean chlorotic mottle caulimovirus (SoCMV), but is not similar to the nonconserved ORF VII in other caulimoviruses. The sequence complementary to a portion of tRNA(Met), thought to be essential for the priming of minus-strand DNA synthesis in caulimoviruses, is located within the coding sequence of ORF A. To explore the functional significance of ORFs VII, A, B, and C, various mutations were engineered into an infectious DNA clone of PCISV. ORFs VII and B are shown to be dispensable, while ORFs A and C are essential. ORF C is a possible functional equivalent of gene III in other caulimoviruses. Sequences within ORF A that are required for efficient priming of minus-strand synthesis are likely to extend beyond the 12-bp tRNA-binding site. Complete deletion of ORF VII was correlated with severe symptoms, notably with the necrosis of apical meristems. Significance of these observations for the understanding of replication and pathogenesis of plant pararetroviruses and for the improvement of caulimovirus-based expression vectors is discussed.
AuthorsA R Mushegian, J A Wolff, R D Richins, R J Shepherd
JournalVirology (Virology) Vol. 206 Issue 2 Pg. 823-34 (Feb 01 1995) ISSN: 0042-6822 [Print] United States
PMID7531917 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA Primers
  • DNA, Viral
  • Trans-Activators
  • RNA-Directed DNA Polymerase
Topics
  • Arachis (virology)
  • Base Sequence
  • Capsid (genetics)
  • Caulimovirus (genetics, metabolism, pathogenicity)
  • Cloning, Molecular
  • DNA Primers
  • DNA, Viral (genetics, isolation & purification)
  • Genes, Lethal
  • Genome, Viral
  • Molecular Sequence Data
  • Mutagenesis
  • Mutagenesis, Insertional
  • Open Reading Frames
  • Plant Diseases
  • Plasmids
  • Polymerase Chain Reaction
  • RNA-Directed DNA Polymerase (genetics)
  • Restriction Mapping
  • Sequence Deletion
  • Trans-Activators (genetics)
  • Virion (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: