Evaluation of a rodent peroxisome proliferator in two species of freshwater fish: rainbow trout (Onchorynchus mykiss) and Japanese medaka (Oryzias latipes)

Abstract

Rainbow trout (Onchorynchus mykiss) and Japanese medaka (Oryzias latipes) were exposed to the hypolipidemic drug gemfibrozil, a known rodent peroxisome proliferator. Trout were injected (i.p.) daily for 2 weeks at doses of 0, 46, 87, or 152 mg/kg/day. Medaka were exposed to the nominal concentrations of 0, 1.25, 2.5, or 5 ppm in water for 2 weeks in a static-renewal system. Peroxisome proliferation was assessed by measuring fatty acyl-CoA oxidase (FAO) activity, peroxisomal bifunctional enzyme (PBE) quantity, and changes in liver-to-body weight ratios (LWR). Results indicate that a mild peroxisome proliferative response was observed in rainbow trout (significant increases in FAO activity at all dose levels and in LWR at the highest dose level). Medaka demonstrated a significant increase in PBE at the highest dose level, while nonsignificant increases in FAO activity were observed at the mid- and high-dose levels.

• Authors: L J Scarano, E J Calabrese, P T Kostecki, L A Baldwin, D A Leonard
• Journal: Ecotoxicology and environmental safety (Ecotoxicol Environ Saf) Vol. 29 Issue 1 Pg. 13-9 (Oct 1994) ISSN: 0147-6513 [Print] Netherlands
• PMID: 7529160 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Water Pollutants, Chemical
• Gemfibrozil

Topics

• Animals
• Body Weight (drug effects)
• Female
• Gemfibrozil (pharmacology)
• Injections, Intraperitoneal
• Liver (drug effects)
• Male
• Microbodies (drug effects, physiology)
• Oncorhynchus mykiss
• Organ Size (drug effects)
• Oryzias
• Rodentia
• Water Pollutants, Chemical (pharmacology)