Primitive multilineage progenitor cells predominate in peripheral blood early after mobilization with high-dose cyclophosphamide and GM-CSF or G-CSF.

Abstract

The change in phenotype, number and proliferative capacity of peripheral blood hematopoietic progenitors (PBHP) was studied in six patients with multiple myeloma during hematopoietic recovery after mobilization with high-dose cyclophosphamide and GM-CSF or G-CSF. In all six patients the first CD34+ cells appearing in the peripheral blood (PB) after cytoreductive treatment were predominantly CD34+/33- (> 70%). At later stages when leukapheresis procedures were started, the CD34+/33+ cells predominated in five of six patients. In leukapheresis harvests of peripheral blood, and in bone marrow addition of SCF and IL-6 to the culturing medium enhanced the plating efficiency. In peripheral blood an increase from 12 to 22% for CD34+/33+ and from 6 to 14% for CD34+/33- was observed. In normal bone marrow we observed an increase from 15 to 23% for CD34+/33+ and from 7 to 17% for CD34+/33-. Highly proliferative progenitors (>500 cells) in the CD34+/33- fraction appeared to be dependent on the addition of 'stem cell recruiting factors' (SCF and IL-6); in bone marrow the percentage of wells with >500 cells increased from 0.9 to 12.6% after SCF+IL-6 and in PBHP from 2 to 9%. We conclude that the first progenitors appearing in the peripheral blood after priming with high-dose cyclophosphamide and GM- or G-CSF have a more primitive immunophenotype, CD34+/33-.

• Authors: S Croockewit, R Raymakers, C Trilsbeek, H Dolstra, A Pennings, T de Witte
• Journal: Leukemia (Leukemia) Vol. 8 Issue 12 Pg. 2194-9 (Dec 1994) ISSN: 0887-6924 [Print] England
• PMID: 7528861 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• Antigens, CD34
• Antigens, Differentiation, Myelomonocytic
• CD33 protein, human
• Hematopoietic Cell Growth Factors
• Interleukin-6
• Sialic Acid Binding Ig-like Lectin 3
• Stem Cell Factor
• Granulocyte Colony-Stimulating Factor
• Granulocyte-Macrophage Colony-Stimulating Factor
• Cyclophosphamide

Topics

• Adult
• Antigens, CD (analysis)
• Antigens, CD34
• Antigens, Differentiation, Myelomonocytic (analysis)
• Bone Marrow (pathology)
• Colony-Forming Units Assay
• Cyclophosphamide (administration & dosage)
• Female
• Flow Cytometry
• Granulocyte Colony-Stimulating Factor (therapeutic use)
• Granulocyte-Macrophage Colony-Stimulating Factor (therapeutic use)
• Hematopoietic Cell Growth Factors (pharmacology)
• Hematopoietic Stem Cells (pathology)
• Humans
• Immunophenotyping
• Interleukin-6 (pharmacology)
• Leukapheresis
• Male
• Middle Aged
• Multiple Myeloma (blood, drug therapy, therapy)
• Sialic Acid Binding Ig-like Lectin 3
• Stem Cell Factor