Characterization of a secreted form of measles virus haemagglutinin expressed from a vaccinia virus recombinant.

The measles virus (MV) haemagglutinin (HA) is a class 2 glycoprotein by means of which the virus particle attaches to the host cell receptor. We have previously expressed this glycoprotein as a vaccinia recombinant virus and have shown that the HA glycoprotein synthesized is indistinguishable from that coded by MV. In the present study, we report that in RK13 cells a soluble form (sHA) of the HA is secreted into the medium. We show by SDS-PAGE and sucrose density gradient centrifugation that the sHA is a dimer and is smaller than the cell-associated form. Using a variety of inhibitors the production of sHA was shown to be a late event, probably occurring at the membrane; only fully glycosylated molecules were found in sHA. Finally, we demonstrate that sHA retains its antigenicity with conformation-dependent MAbs and its receptor recognition function. We conclude that sHA is a valuable tool for use in studies of the structure and function of the MV HA glycoprotein.
AuthorsE Malvoisin, F Wild
JournalThe Journal of general virology (J Gen Virol) Vol. 75 ( Pt 12) Pg. 3603-9 (Dec 1994) ISSN: 0022-1317 [Print] ENGLAND
PMID7527839 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigens, CD
  • Antigens, CD46
  • Antigens, Viral
  • Epitopes
  • Hemagglutinins, Viral
  • Membrane Glycoproteins
  • Protein Synthesis Inhibitors
  • Receptors, Virus
  • Recombinant Proteins
  • hemagglutinin protein G, measles virus
  • Hexosaminidases
  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigens, CD (metabolism)
  • Antigens, CD46
  • Antigens, Viral (metabolism)
  • Biological Transport
  • Cell Line
  • Cloning, Molecular
  • Epitopes (analysis)
  • Glycosylation
  • Hemagglutinins, Viral (biosynthesis, chemistry, genetics, metabolism)
  • Hexosaminidases
  • Measles virus (chemistry, metabolism)
  • Membrane Glycoproteins (metabolism)
  • Molecular Weight
  • Protein Conformation
  • Protein Synthesis Inhibitors
  • Receptors, Virus (metabolism)
  • Recombinant Proteins (biosynthesis, chemistry, metabolism)
  • Solubility
  • Vaccinia virus (genetics)

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