HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Characterization of a secreted form of measles virus haemagglutinin expressed from a vaccinia virus recombinant.

Abstract
The measles virus (MV) haemagglutinin (HA) is a class 2 glycoprotein by means of which the virus particle attaches to the host cell receptor. We have previously expressed this glycoprotein as a vaccinia recombinant virus and have shown that the HA glycoprotein synthesized is indistinguishable from that coded by MV. In the present study, we report that in RK13 cells a soluble form (sHA) of the HA is secreted into the medium. We show by SDS-PAGE and sucrose density gradient centrifugation that the sHA is a dimer and is smaller than the cell-associated form. Using a variety of inhibitors the production of sHA was shown to be a late event, probably occurring at the membrane; only fully glycosylated molecules were found in sHA. Finally, we demonstrate that sHA retains its antigenicity with conformation-dependent MAbs and its receptor recognition function. We conclude that sHA is a valuable tool for use in studies of the structure and function of the MV HA glycoprotein.
AuthorsE Malvoisin, F Wild
JournalThe Journal of general virology (J Gen Virol) Vol. 75 ( Pt 12) Pg. 3603-9 (Dec 1994) ISSN: 0022-1317 [Print] ENGLAND
PMID7527839 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigens, CD
  • Antigens, CD46
  • Antigens, Viral
  • Epitopes
  • Hemagglutinins, Viral
  • Membrane Glycoproteins
  • Protein Synthesis Inhibitors
  • Receptors, Virus
  • Recombinant Proteins
  • hemagglutinin protein G, measles virus
  • Hexosaminidases
Topics
  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigens, CD (metabolism)
  • Antigens, CD46
  • Antigens, Viral (metabolism)
  • Biological Transport
  • Cell Line
  • Cloning, Molecular
  • Epitopes (analysis)
  • Glycosylation
  • Hemagglutinins, Viral (biosynthesis, chemistry, genetics, metabolism)
  • Hexosaminidases
  • Measles virus (chemistry, metabolism)
  • Membrane Glycoproteins (metabolism)
  • Molecular Weight
  • Protein Conformation
  • Protein Synthesis Inhibitors
  • Receptors, Virus (metabolism)
  • Recombinant Proteins (biosynthesis, chemistry, metabolism)
  • Solubility
  • Vaccinia virus (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: