Genotoxicity assessment of aromatic amines and amides in genetically engineered V79 cells.

Abstract

A genetically engineered V79 cell line expressing rat CYP1A2 and another cell line expressing rat CYP1A2 as well as endogenous acetyltransferase activity, as well as CYP-deficient parental V79 cell lines, were used to assess the genotoxicity of the aromatic amines and amides 2-aminoanthracene, 2-aminofluorene, 2-acetylaminofluorene, 4-acetylaminofluorene and 2-amino-3-methylimidazo[4,5-f]quinoline, with chromosomal aberrations and sister chromatid exchanges as the end-points. None of the test compounds showed a clear effect on the frequency of chromosomal aberrations in any cell line used. Sister chromatid exchanges, however, were induced by 2-aminoanthracene, 2-aminofluorene and 2-acetylaminofluorene in the CYP1A2-proficient cells, but not in the CYP1A2-deficient cells. The presence of acetyltransferase activity enhanced the effect of 2-aminoanthracene, 2-aminofluorene and 2-acetylaminofluorene. 4-Acetylaminofluorene and 2-amino-3-methylimidazo[4,5-f]quinoline did not induce sister chromatid exchanges in the investigated cell lines. The use of cell lines with defined metabolic capabilities seems to be a valuable tool to study specific metabolic pathways important in the activation of procarcinogens.

• Authors: A S Rodrigues, I D Silva, M H Caria, A Laires, T Chaveca, H R Glatt, J Rueff
• Journal: Mutation research (Mutat Res) Vol. 341 Issue 2 Pg. 93-100 (Dec 1994) ISSN: 0027-5107 [Print] Netherlands
• PMID: 7527492 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anthracenes
• Fluorenes
• Mutagens
• Quinolines
• 2-amino-3-methylimidazo(4,5-f)quinoline
• 2-aminofluorene
• 2-anthramine
• Cytochrome P-450 Enzyme System
• 2-Acetylaminofluorene
• Oxidoreductases
• Cytochrome P-450 CYP1A2
• 4-acetylaminofluorene

Topics

• 2-Acetylaminofluorene (analogs & derivatives, toxicity)
• Animals
• Anthracenes (toxicity)
• Biotransformation
• Cell Line
• Chromosome Aberrations
• Cricetinae
• Cytochrome P-450 CYP1A2
• Cytochrome P-450 Enzyme System (genetics, physiology)
• Fluorenes (toxicity)
• Genetic Engineering
• Mutagenicity Tests
• Mutagens (metabolism, toxicity)
• Oxidoreductases (genetics, physiology)
• Quinolines (toxicity)
• Rats
• Sister Chromatid Exchange (drug effects)