Expression and down-regulation by retinoic acid of IGF binding protein-2 and -4 in medium from human neuroblastoma cells.

Abstract

Insulin-like growth factors (IGFs) regulate the autocrine/paracrine growth of neuroblastomas. The IGFs bind to specific binding proteins (IGFBPs) which modulate their biological activity. We investigated, by Western ligand blotting (WLB), the presence of IGFBPs and their possible modulation by retinoic acid (RA), IGF-I, IGF-II and truncated Des(1-3)IGF-I in conditioned medium (CM) of the human neuroblastoma SK-N-BE(2) cell line. We demonstrated the presence of two IGFBPs, with MW 37 kDa and 25 kDa. Following immunoprecipitation, they turned out to be IGFBP-2 and -4, respectively. The RA-induced differentiation in SK-N-BE(2) cells was accompanied by a marked reduction of the intensity of both IGFBP bands after 48 h (32% and 24% of control, respectively) and 72 h (2% and 0% of control, respectively) incubation. The addition of exogenous IGFs, which did not induce cell differentiation, did not change the IGFBP pattern significantly, except for the truncated form of IGF-I, which induced a marked decrease in both the 37 kDa and 25 kDa bands after 72 h incubation (45% and 18% of control, respectively). These findings suggest that IGFBPs have a role in RA-induced differentiation in human neuroblastoma cells.

• Authors: S Bernardini, S Cianfarani, A Spagnoli, M Annicchiarico-Petruzzelli, G Melino, R Massoud, B Boscherini, A Finazzi-Agró, R G Rosenfeld, G Federici
• Journal: Journal of neuroendocrinology (J Neuroendocrinol) Vol. 6 Issue 4 Pg. 409-13 (Aug 1994) ISSN: 0953-8194 [Print] United States
• PMID: 7527270 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carrier Proteins
• Insulin-Like Growth Factor Binding Protein 2
• Insulin-Like Growth Factor Binding Protein 4
• Insulin-Like Growth Factor Binding Proteins
• Tretinoin

Topics

• Blotting, Western
• Carrier Proteins (metabolism)
• Cell Differentiation (drug effects)
• Down-Regulation
• Humans
• Immunosorbent Techniques
• Insulin-Like Growth Factor Binding Protein 2
• Insulin-Like Growth Factor Binding Protein 4
• Insulin-Like Growth Factor Binding Proteins
• Neurites (drug effects)
• Neuroblastoma (metabolism, ultrastructure)
• Tretinoin (pharmacology)
• Tumor Cells, Cultured