Expression of the ras-related rab3a gene in human insulinomas and normal human pancreatic islets.

Abstract

To determine the expression of the small-molecular-weight guanosine 5'-triphosphate (GTP)-binding protein rab3a in human endocrine pancreatic tissue, total RNA was isolated from five different human insulinomas and from normal human islets. The expression of rab3a was analyzed by Northern blots utilizing a 1,300-bp Eco RI fragment of the cDNA coding for human rab3a. As in the brain, a specific transcript of 1,800 bp was detected in all insulinomas, though with varying signal intensity. When the northern membranes were rehybridized with a radioactively labeled insulin probe, a signal was found for all five insulinomas and cells of normal islets. In contrast, normal human islets showed no detectable expression of rab3a. In conclusion, rab3a is expressed to a significant degree in human insulinoma tissue but not in normal islets.

• Authors: B Lankat-Buttgereit, H C Fehmann, B J Hering, R G Bretzel, B Göke
• Journal: Pancreas (Pancreas) Vol. 9 Issue 4 Pg. 434-8 (Jul 1994) ISSN: 0885-3177 [Print] United States
• PMID: 7524063 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• RNA, Neoplasm
• RNA
• GTP-Binding Proteins
• rab3 GTP-Binding Proteins

Topics

• Animals
• Blotting, Northern
• GTP-Binding Proteins (biosynthesis, genetics)
• Gene Expression
• Genes, ras (genetics)
• Humans
• Insulinoma (genetics)
• Islets of Langerhans (metabolism)
• Pancreatic Neoplasms (genetics)
• RNA (analysis, genetics, isolation & purification)
• RNA, Neoplasm (analysis, genetics, isolation & purification)
• Rats
• rab3 GTP-Binding Proteins