Recruitment of pathogenic T cells to synovial tissues of rats injected intraarticularly with nonspecific agents.

Abstract

To study a role for T cells in nonspecifically induced arthritis, rats were injected intraarticularly into ankle joints with mineral oils including 2,6,10,15,19,23-hexamethyltetracosane (squalene) and incomplete Freund's adjuvant. The results showed that moderate joint inflammation had developed by Day 6 after the nonspecific stimulation. This was then followed by induction of severe arthritis, reaching a peak on Day 21. Histologically, the early phase of arthritis was associated with edema of synovial tissues containing many polymorphs and monocytes/macrophages and the late phase of the joint inflammation with marked hyperplasia of synovial membrane with dense infiltration of CD5+ and alpha beta+ T cells. Depletion of alpha beta+ T cells by a monoclonal antibody against TCR alpha beta suppressed both induction and progression of the late phase of arthritis. Thus, pathogenic T cells appear to be recruited to joint tissues following nonspecific stimulation.

• Authors: S Yoshino, J Yoshino
• Journal: Cellular immunology (Cell Immunol) Vol. 158 Issue 2 Pg. 305-13 (Oct 15 1994) ISSN: 0008-8749 [Print] Netherlands
• PMID: 7522973 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antigens, CD
• CD5 Antigens
• Receptors, Antigen, T-Cell, alpha-beta
• Squalene
• Collagen

Topics

• Animals
• Antibodies, Monoclonal (therapeutic use)
• Antigens, CD (immunology)
• Arthritis, Experimental (immunology, pathology, therapy)
• CD5 Antigens
• Collagen (immunology)
• Female
• Immunoenzyme Techniques
• Injections, Intra-Articular
• Rats
• Rats, Inbred Strains
• Receptors, Antigen, T-Cell, alpha-beta (immunology)
• Squalene (immunology)
• Synovial Membrane (immunology)
• T-Lymphocyte Subsets (immunology)
• T-Lymphocytes, Cytotoxic (immunology)