Characterization of the Spodoptera frugiperda TATA-binding protein: nucleotide sequence and response to baculovirus infection.

Abstract

A cDNA clone containing a 921 bp open-reading frame (307 amino acids; 34 kDa) homologous to the TATA-binding protein (TBP) was isolated and sequenced from a Spodoptera frugiperda cell line that is commonly used in the baculovirus expression system. Analysis of the S. frugiperda TBP (SfTBP) sequence showed that the amino-terminal portion of SfTBP diverged significantly from that of other TBP sequences including Drosophila melanogaster whereas the carboxy-terminal sequence was highly conserved. Southern blot analysis indicated that SfTBP was encoded by a single gene in the S. frugiperda genome. Northern blot analysis indicated that steady-state levels of the 1.3 kb SfTBP transcript declined by 24 h post-infection corresponding to the time of virus-induced inhibition of host-cell transcription. Corresponding western blot analysis showed that TBP protein levels remain constant up to 72 h post-infection.

• Authors: C Rasmussen, G F Rohrmann
• Journal: Insect biochemistry and molecular biology (Insect Biochem Mol Biol) Vol. 24 Issue 7 Pg. 699-708 (Jul 1994) ISSN: 0965-1748 [Print] England
• PMID: 7520800 (Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA Primers
• DNA-Binding Proteins
• TATA-Box Binding Protein
• Transcription Factors
• RNA

Topics

• Amino Acid Sequence
• Animals
• Baculoviridae (physiology)
• Base Sequence
• Blotting, Northern
• Cell Line
• Cloning, Molecular
• DNA Primers
• DNA-Binding Proteins (biosynthesis, genetics)
• Drosophila melanogaster (genetics, metabolism)
• Genetic Variation
• Genetic Vectors
• Molecular Sequence Data
• Moths (genetics, metabolism, microbiology)
• Open Reading Frames
• Phylogeny
• Polymerase Chain Reaction
• RNA (analysis, biosynthesis)
• Sequence Homology, Amino Acid
• TATA Box
• TATA-Box Binding Protein
• Transcription Factors (biosynthesis, genetics)
• Transcription, Genetic