Immunohistochemistry of port-wine stains and normal skin with endothelium-specific antibodies PAL-E, anti-ICAM-1, anti-ELAM-1, and anti-factor VIIIrAg.

Abstract

BACKGROUND AND DESIGN:

Immunohistochemical analysis using four monoclonal antibodies specific for endothelium was performed to evaluate the possible role the endothelium may play in the pathogenesis of port-wine stains. In 11 patients with port-wine stains, biopsy specimens were obtained from involved and normal skin. On frozen tissue sections, we studied and compared the distribution and staining pattern of PAL-E, anti-intercellular adhesion molecule-1 (ICAM-1), anti-endothelial leukocyte adhesion molecule-1 (ELAM-1), and anti-factor VIIIrAg (FVIIIrAg), all recognizing specific epitopes of vascular endothelial cells.

RESULTS:

The PAL-E, anti-FVIIIrAg, and anti-ICAM-1 antibodies showed a similar distribution and staining pattern. The intensity of staining was equally strong with PAL-E and FVIIIrAg, while the expression of ICAM-1 was moderate. The ELAM-1 antibody exhibited only a weak expression in about 70% of evaluated specimens. No substantial differences in the intensity and distribution pattern of expression of these proteins could be demonstrated between normal skin and port-wine stains.

CONCLUSION:

Our findings suggest that the abnormal vessel pathologic findings in port-wine stains are not due to defects associated with the endothelium. According to PAL-E antibody staining properties, port-wine stain vessels could be classified as capillaries and/or postcapillary venules and small veins.

• Authors: R Neumann, H Leonhartsberger, R Knobler, H Hönigsmann
• Journal: Archives of dermatology (Arch Dermatol) Vol. 130 Issue 7 Pg. 879-83 (Jul 1994) ISSN: 0003-987X [Print] United States
• PMID: 7517655 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antigens, CD
• Cell Adhesion Molecules
• E-Selectin
• Receptors, Immunologic
• von Willebrand Factor
• Intercellular Adhesion Molecule-1

Topics

• Adult
• Aged
• Antibodies, Monoclonal (immunology)
• Antigens, CD (immunology)
• Cell Adhesion Molecules (immunology)
• E-Selectin
• Endothelium (immunology)
• Female
• Hamartoma (immunology, pathology)
• Humans
• Immunohistochemistry
• Intercellular Adhesion Molecule-1
• Male
• Middle Aged
• Receptors, Immunologic (immunology)
• Skin Diseases (immunology, pathology)
• von Willebrand Factor (immunology)