Plasmodium falciparum: pfalhesin and CD36 form an adhesin/receptor pair that is responsible for the pH-dependent portion of cytoadherence/sequestration.

Abstract

The cytoadherent behavior of two Plasmodium falciparum (human malaria) cell lines, FCR-3 and ITO4 (a cell line with elevated ICAM-1 adherence), was studied using CHO cells transfected with CD36 or ICAM-1 receptors as target cells. ICAM-1-mediated adherence was found to be relatively pH insensitive, whereas CD36-mediated adherence was pH sensitive and inhibited by monoclonal antibodies and peptides based on a region found in human band 3 protein and named pfalhesin. Immobilized pfalhesin was used as an affinity matrix to purify CD36 from extracts of C32 amelanotic melanoma cells, which have ICAM-1 as well as CD36 receptors, and bind both parasite cell lines. We conclude that pfalhesin and CD36 constitute an adhesin/receptor pair.

• Authors: I Crandall, K M Land, I W Sherman
• Journal: Experimental parasitology (Exp Parasitol) Vol. 78 Issue 2 Pg. 203-9 (Mar 1994) ISSN: 0014-4894 [Print] United States
• PMID: 7509756 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Anion Exchange Protein 1, Erythrocyte
• Antigens, CD
• CD36 Antigens
• Cell Adhesion Molecules
• pfalhesin
• Intercellular Adhesion Molecule-1

Topics

• Amino Acid Sequence
• Animals
• Anion Exchange Protein 1, Erythrocyte (physiology)
• Antigens, CD (physiology)
• CD36 Antigens
• CHO Cells
• Cell Adhesion
• Cell Adhesion Molecules (physiology)
• Cricetinae
• Erythrocytes (parasitology)
• Humans
• Hydrogen-Ion Concentration
• Immunoblotting
• Intercellular Adhesion Molecule-1
• Melanoma, Amelanotic
• Molecular Sequence Data
• Plasmodium falciparum (physiology)
• Tumor Cells, Cultured