Abstract |
The adhesion molecules E-selectin (ELAM-1) and P-selectin (GMP-140/CD62) recognize the carbohydrate motives sialyl-Le(x), sialyl-diLe(x), or sialyl-Lea, though with different affinity. We found that the melanoma cell line NKI-4 bound to E-selectin, but not to P-selectin. This melanoma cell line did not express sialyl-Le(x), but was positive for sialyl-diLe(x) and sialyl-Le(a). In contrast, 2 other melanoma cell lines, MeWo and SK-MEL-28, expressing either sialyl-diLe(x) or sialyl-Le(a) on the cell surface, bound neither E-selectin nor P-selectin. Transfection of the fucosyltransferases Fuc-TIII, Fuc-TIV, and Fuc-TV mediates cell surface expression of sialyl-Le(x) in many cell lines. We detected transcripts of the fucosyltransferases Fuc-TIII and Fuc-TV in 4 melanoma cell lines despite the absence of cell surface sialyl-Le(x). Our observations indicate that expression of fucosyltransferases ( Fuc-TIII and -TV) and generation of cell-surface sialyl-diLe(x) are not sufficient to permit adherence to E-selectin or P-selectin. Furthermore, it seems possible that a yet undefined ligand different from sialyl-Le(x), sialyl-diLe(x), or sialyl-Le(a) enables melanoma cells to adhere to E-selectin.
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Authors | U Kunzendorf, S Krüger-Krasagakes, M Notter, H Hock, G Walz, T Diamantstein |
Journal | Cancer research
(Cancer Res)
Vol. 54
Issue 4
Pg. 1109-12
(Feb 15 1994)
ISSN: 0008-5472 [Print] United States |
PMID | 7508820
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cell Adhesion Molecules
- E-Selectin
- Lewis X Antigen
- P-Selectin
- Platelet Membrane Glycoproteins
- RNA, Messenger
- Fucosyltransferases
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Topics |
- Base Sequence
- Cell Adhesion
- Cell Adhesion Molecules
(metabolism)
- E-Selectin
- Flow Cytometry
- Fucosyltransferases
(genetics)
- Humans
- Lewis X Antigen
(analysis)
- Melanoma
(metabolism, pathology)
- Molecular Sequence Data
- P-Selectin
- Platelet Membrane Glycoproteins
(metabolism)
- Polymerase Chain Reaction
- RNA, Messenger
(analysis)
- Tumor Cells, Cultured
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