Changes in cell adhesion molecule expression on T cells associated with systemic virus infection.

Abstract

Virus-induced changes in adhesion molecule expression on T cells were investigated to understand how antiviral effector cells migrate into infectious foci. FACS analysis revealed that after systemic infection with lymphocytic choriomeningitis virus a number of cell adhesion molecules, including VLA-4, LFA-1, and ICAM-1, are up-regulated on CD8+ cells, whereas the lymph node homing receptor MEL-14 is down-regulated during the infection; only marginal changes were observed for CD4+ cells. Basically similar but less marked results were obtained in mice infected with Pichinde virus. Further analyses showed that T cells with a changed adhesion molecule profile tended to present other cell surface markers indicating a state of cellular activation, e.g., IL-2R, and included all virus-specific CTL effectors. Regarding the physiologic significance of these changes in adhesion molecule expression, it was found that up-regulation of VLA-4 expression on splenic T cells correlated with influx of inflammatory cells into the cerebrospinal fluid of intracerebrally infected animals, and that the number of CD8+VLA-4hi cells increased from lymph nodes and spleen to blood and cerebrospinal fluid. These results support the hypothesis that up-regulation of VLA-4 is important for effector T cell homing to sites of inflammation.

• Authors: E C Andersson, J P Christensen, O Marker, A R Thomsen
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 152 Issue 3 Pg. 1237-45 (Feb 01 1994) ISSN: 0022-1767 [Print] United States
• PMID: 7507962 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CD8 Antigens
• Cell Adhesion Molecules
• Lymphocyte Function-Associated Antigen-1
• Receptors, Very Late Antigen
• Intercellular Adhesion Molecule-1
• L-Selectin

Topics

• Animals
• CD8 Antigens (analysis)
• Cell Adhesion Molecules (metabolism)
• Cerebrospinal Fluid (immunology)
• Cytotoxicity, Immunologic
• Female
• Flow Cytometry
• Intercellular Adhesion Molecule-1
• L-Selectin
• Lymphocyte Activation
• Lymphocyte Function-Associated Antigen-1 (metabolism)
• Lymphocytic Choriomeningitis (immunology)
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C3H
• Mice, Inbred C57BL
• Receptors, Very Late Antigen (metabolism)
• T-Lymphocyte Subsets (cytology, immunology)
• T-Lymphocytes, Cytotoxic (immunology)