Modulation of protectin (CD59 antigen) cell surface expression on human neoplastic cell lines.

Abstract

The ability of cytokines (IFN alpha, IFN gamma, TNF alpha, IL-1 alpha, IL-6), all-trans retinoic acid, 1,25(OH)2-vitamin D3 and the tumor promoting phorbol ester TPA to regulate cell surface expression of protectin (CD59 antigen) on human hematopoietic and non-hematopoietic neoplastic cell lines was examined with the aid of immunocytofluorometric measurements. The tumor promoting phorbol ester TPA induced a marked up-regulation of protectin in all examined cell lines with the exception of promyelocytic leukemia HL-60, where TPA significantly decreased protectin cell surface expression. All-trans retinoic acid weakly down-regulated cell surface protectin on K-562, while 1,25(OH)2-vitamin D3 produced such effect on HL-60 cells. None of the examined cytokines induced a significant protectin down-regulation in the examined cell lines.

• Authors: J Sedlák, L Hunáková, J Duraj, M Grófová, B Chorváth
• Journal: Neoplasma (Neoplasma) Vol. 40 Issue 6 Pg. 337-40 ( 1993) ISSN: 0028-2685 [Print] Slovakia
• PMID: 7507222 (Publication Type: Journal Article)

Chemical References

• Antigens, CD
• CD59 Antigens
• Cytokines
• Membrane Glycoproteins
• Tretinoin
• Calcitriol
• Tetradecanoylphorbol Acetate

Topics

• Antigens, CD (biosynthesis)
• Breast Neoplasms (immunology, pathology)
• CD59 Antigens
• Calcitriol (pharmacology)
• Carcinoma (immunology, pathology)
• Cell Membrane (immunology)
• Cytokines (pharmacology)
• Down-Regulation (drug effects)
• Electrophoresis, Polyacrylamide Gel
• Female
• Flow Cytometry
• Fluorescent Antibody Technique
• Glioma (immunology, pathology)
• Humans
• Leukemia, Myeloid (immunology, pathology)
• Leukemia, Promyelocytic, Acute (immunology, pathology)
• Membrane Glycoproteins (biosynthesis)
• Ovarian Neoplasms (immunology, pathology)
• Tetradecanoylphorbol Acetate (pharmacology)
• Tretinoin (pharmacology)
• Tumor Cells, Cultured
• Up-Regulation (drug effects)