Modification of radiation-induced chromosome damage and micronucleus induction in mouse bone marrow by misonidazole and hyperthermia.

Abstract

The effect of misonidazole (MISO), local hyperthermia (HT) and their combination on radiation-induced chromosome damage and micronucleus (MN) induction was studied in mouse bone marrow cells. It was found that MISO treatment did not enhance the clastogenic effect of radiation, which indicates a lack of radiosensitization of bone marrow chromosomes. But post-irradiation HT increased the frequency of aberrant cells and MN. A combination of MISO and HT produced a significant increase in the frequency of radiation-induced aberrant cells and MN at all the radiation doses as compared to radiation alone. The percentage of aberrant cells as well as the percentage of MN showed a linear quadratic increase with radiation dose in all the treatment groups. At higher radiation doses, cells with > 1 MN increased quadratically with a pronounced increase in cells bearing > 2 MN and severely damaged cells (SDCs) at radiation doses above 3.0 Gy in the HT and MISO+HT treated groups. Our results indicate that though MISO itself may not have a radiosensitizing effect on mouse chromosomes, a combination of MISO with HT can enhance the radiation damage in normal bone marrow.

• Authors: K S Bisht, P U Uma Devi
• Journal: Acta oncologica (Stockholm, Sweden) (Acta Oncol) Vol. 34 Issue 7 Pg. 913-8 ( 1995) ISSN: 0284-186X [Print] England
• PMID: 7492380 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Radiation-Sensitizing Agents
• Misonidazole

Topics

• Animals
• Bone Marrow (drug effects, radiation effects)
• Chromosome Aberrations
• Dose-Response Relationship, Radiation
• Hyperthermia, Induced
• Male
• Mice
• Mice, Inbred Strains
• Micronucleus Tests
• Misonidazole (pharmacology)
• Radiation-Sensitizing Agents (pharmacology)
• Whole-Body Irradiation