Differential effect of 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7) on alpha B-crystallin and hsp70 gene expression in murine cell lines.

Abstract	We studied the effect of isoquinolinesulfonamide derivatives (H-7, H-8, and HA1004) on the expression of two heat shock genes (alpha beta-crystallin and hsp70) in NIH 3T3 and Swiss 3T3 cells after heat shock at 45 degrees for 10 min. Western blots and northern blots showed that H-7 effectively suppressed the accumulation of HSP70 and alpha B-crystallin mRNA as well as the synthesis of their proteins. The degree of suppression was dependent upon the concentration of the drug. Moreover, the expression of the hsp genes was differentially suppressed by H-7. The expression of the alpha B-crystallin gene was more effectively inhibited than that of the hsp70 gene by H-7. Nuclear run-on assay demonstrates that this difference was due to the differential effect of H-7 on the elongation of transcription of different hsp genes.
Authors	Y J Lee, C M Berns, S Galoforo, G Erdos, J M Cho, P M Corry
Journal	Biochemical pharmacology (Biochem Pharmacol) Vol. 50 Issue 8 Pg. 1149-55 (Oct 12 1995) ISSN: 0006-2952 [Print] England
PMID	7488228 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References	Crystallins Enzyme Inhibitors HSP70 Heat-Shock Proteins Isoquinolines Piperazines RNA, Messenger Sulfonamides 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine N-(2-guanidinoethyl)-5-isoquinolinesulfonamide Protein Kinase C
Topics	1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine 3T3 Cells Animals Base Sequence Cell Line Crystallins (biosynthesis, genetics) Enzyme Inhibitors (pharmacology) Gene Expression (drug effects) HSP70 Heat-Shock Proteins (biosynthesis, genetics) Isoquinolines (pharmacology) Mice Molecular Sequence Data Piperazines (pharmacology) Protein Kinase C (antagonists & inhibitors) RNA, Messenger (biosynthesis) Sulfonamides Transcription, Genetic (drug effects)

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