Abstract |
The cardiac L-type voltage-dependent calcium channel ( VDCC) is a critical component of cardiac action potential and excitation-contraction coupling. The objective of the present study was to examine the changes in expression in Motif IV, an alternatively spliced region of the alpha-1 subunit of the VDCC channel in postmyocardial infarction (MI) remodeled rat left ventricle. RNase protection assay was used to determine alteration in isoform expression in the noninfarcted hypertrophied ventricular myocardium 21 days post myocardial infarction. Our study demonstrates that cardiac hypertrophy is associated with significant increase in the mRNA level of the fetal isoform, with the reversion of fetal:adult isoform ratio to the fetal phenotype. Changes in isoform expression in the post-MI remodeled ventricle, not previously reported, is a pertinent genetic marker of cardiac hypertrophy.
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Authors | M Gidh-Jain, B Huang, P Jain, V Battula, N el-Sherif |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 216
Issue 3
Pg. 892-7
(Nov 22 1995)
ISSN: 0006-291X [Print] United States |
PMID | 7488209
(Publication Type: Journal Article)
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Chemical References |
- Calcium Channels
- RNA, Messenger
- Ribonucleases
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Topics |
- Alternative Splicing
- Animals
- Base Sequence
- Calcium Channels
(genetics)
- Female
- Fetus
(metabolism)
- Gene Expression
- Hypertrophy, Left Ventricular
(metabolism)
- Molecular Sequence Data
- Myocardial Infarction
(metabolism)
- Myocardium
(metabolism)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Ribonucleases
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