Detection of superoxide free radicals in rats with acute pancreatitis.

Abstract

To study the importance of oxygen-derived free radicals in acute pancreatitis, an experimental study of in vivo detection of superoxide free radicals (O2-) was performed using rats. Using a new chemiluminescence probe (2-methyl-6-[p-methoxyphenyl]-3,7-dihydro-imidazol[1,2-1]pyrazin- 3-one; MCLA; a Cypridina luciferin analogue) and a highly sensitive photon counting system, O2- from the pancreatic surface of rats with experimental acute pancreatitis induced by 180 micrograms cerulein/kg was detected. The time course of MCLA-dependent luminescence suggested that O2- production began 2-3 h after cerulein injection and then decreased gradually. Superoxide free radical production in the pancreas of rats with cerulein-induced acute pancreatitis was confirmed using MCLA-dependent chemiluminescence. This new method allows direct observation of the behavior of oxygen-derived free radicals.

• Authors: W Kishimoto, A Nakao, M Nakano, A Takahashi, H Inaba, H Takagi
• Journal: Pancreas (Pancreas) Vol. 11 Issue 2 Pg. 122-6 (Aug 1995) ISSN: 0885-3177 [Print] United States
• PMID: 7479667 (Publication Type: Journal Article)

Chemical References

• Free Radicals
• Imidazoles
• Pyrazines
• Superoxides
• 2-methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo(1,2-alpha)pyrazin-3-one
• Ceruletide
• Superoxide Dismutase

Topics

• Acute Disease
• Animals
• Ceruletide
• Free Radicals
• Imidazoles
• Kinetics
• Luminescent Measurements
• Male
• Pancreas (chemistry)
• Pancreatitis (chemically induced, metabolism)
• Pyrazines
• Rats
• Rats, Wistar
• Superoxide Dismutase (metabolism, pharmacology)
• Superoxides (analysis)