Mitsuokella dentalis in human periodontitis.

Abstract

The occurrence of Mitsuokella dentalis in periodontitis was determined by culture and DNA probe detection. Subgingival paper-point samples from 480 periodontitis patients were transported in VMGA III, plated onto brucella agar with 5% sheep blood and incubated anaerobically for 7 days. Presumptive identification was based on a colony morphology resembling a water drop and biochemical characteristics. DNA probe detection was performed on paper-point samples using a digoxigenin-labeled cellular M. dentalis DNA probe in a dot-blot assay. Culture and DNA probe identified M. dentalis in 18.1% and in 80.7% of the study patients, respectively. M. dentalis isolates produced phosphatases, galactosidase, glucosidase and acetylglucosaminidase and showed high in vitro sensitivity to metronidazole. This study revealed that M. dentalis is a constituent of the pathogenic microbiota in human periodontitis. The periodontopathic potential of the organism is unknown.

• Authors: M J Flynn, G Li, J Slots
• Journal: Oral microbiology and immunology (Oral Microbiol Immunol) Vol. 9 Issue 4 Pg. 248-50 (Aug 1994) ISSN: 0902-0055 [Print] Denmark
• PMID: 7478766 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Anti-Bacterial Agents
• DNA Probes
• Penicillins
• Metronidazole
• Ciprofloxacin
• Amoxicillin
• Tetracycline

Topics

• Adolescent
• Adult
• Age Distribution
• Aged
• Amoxicillin (pharmacology)
• Anti-Bacterial Agents (pharmacology)
• Bacteroides (drug effects, isolation & purification, metabolism)
• Ciprofloxacin (pharmacology)
• Cross-Sectional Studies
• DNA Probes
• Drug Resistance, Microbial
• Female
• Humans
• Male
• Metronidazole (pharmacology)
• Microbial Sensitivity Tests
• Middle Aged
• Penicillins (pharmacology)
• Periodontal Pocket (microbiology)
• Periodontitis (microbiology)
• Sex Factors
• Tetracycline (pharmacology)
• Tetracycline Resistance